Isolation and Identification of the Pathogen Causing Stem Rot Disease in Passiflora edulia and Fungicide Screening

doi:10.11924/j.issn.1000-6850.casb2020-0817

Abstract

Abstract:

The paper aims to find out the pathogen which causes stem rot disease in passion fruit, and the chemicals to control this disease effectively. Stem rot diseased samples were collected from different Passiflora edulia production fields in Guangdong. The pathogen was isolated and determined by methods of morphological observation and molecular identification. The strains’ pathogenicity was measured, and their sensibility to eight fungicides was also detected. Results showed that eight isolated pathogen strains were identified as Fusarium solani. Based on in vivo and in vitro tests with Passiflora edulis, strains of FP6, FP7 and FP2 showed highest pathogenicity, followed by FP1, FP3, FP8 and FP4, and the lowest was FP5. Different fungicides showed effects on pathogen growth rate and pathogenicity, and 30% benazoxystrobin showed the best inhibition effects. This study could lay a material and technology base for further research on pathogenic mechanism of stem rot disease, and disease resistance breeding program of passion fruit.

Key words: Passiflora edulia, stem rot disease, fusarium pathogen, pathogenicity, fungicide

CLC Number:

S482

Kuang Ruibin, Yang Hu, Yang Min, Zhou Chenping, Huang Bingxiong, Wei Yuerong. Isolation and Identification of the Pathogen Causing Stem Rot Disease in Passiflora edulia and Fungicide Screening[J]. Chinese Agricultural Science Bulletin, 2021, 37(28): 108-114.

Figures/Tables 8

References 28

[1]	霍丹群, 蒋兰, 马璐璐, 等. 百香果功能研究及其开发进展[J]. 食品工业科技, 2012, 33(19):391-395.
[2]	许海棠, 田程飘, 赵彦芝, 等. 百香果叶总酚的提取工艺优化、抗氧化活性及其抑制α-葡萄糖苷酶活性[J]. 食品工业科技, 2019, 20:223-227,236.
[3]	Luana C S, Raphaela G B, Philipe S, et al. Solubility of passion fruit (Passiflora edulis Sims) seed oil in supercritical CO₂[J]. Fluid Phase Equilibria, 2019, 49(31):174-180.
[4]	Nerdy N, Kiking R. Hepatoprotective activity and nephroprotective activity of peel extract from three varieties of the passion fruit (Passiflora sp.) in the Albino rat[J]. Open access Macedonian Journal of Medical Sciences, 2019, 7(4):536-542. doi: 10.3889/oamjms.2019.153 pmid: 30894908
[5]	余东, 熊丙全, 袁军, 等. 西番莲种质资源概况及其应用研究现状[J]. 中国南方果树, 2005(1):36-37.
[6]	邝瑞彬, 杨护, 孔凡利, 等. 广东省百香果产业现状与发展策略[J]. 广东农业科学, 2019, 46(9):165-172.
[7]	宋晓兵, 崔一平, 彭埃天, 等. 广东西番莲茎基腐病病原的分离及鉴定[J]. 南方农业学报, 2019, 50(5):1007-1012.
[8]	Gardner D E. Pathogenicity of Fusarium oxysporum f. sp. passiflorae to banana poka and other Passiflora spp. in Hawaii[J]. Plant Disease, 1989, 73:476-478. doi: 10.1094/PD-73-0476 URL
[9]	Ploetz R C. Sudden wilt of passion fruit in southern Florida caused by Nectria haematococca[J]. Plant Disease, 1991, 75:1071-1073. doi: 10.1094/PD-75-1071 URL
[10]	Li D F, Yang J Q, Zhang X Y, et al. Identification of the pathogen causing collar rot of passion fruit in Fujian[J]. Acta Hytopathologica Sinica, 1993, 23:372.
[11]	李德福, 杨佳琪, 张晓燕, 等. 福建省西番莲茎基腐病病原鉴定[J]. 植物病理学报, 1993(4):86.
[12]	郑加协, 黄盈, 梁渭州, 等. 福建西番莲茎基腐病的病原菌鉴定[J]. 福建省农科院学报, 1992(2):65-68.
[13]	黄志巧, 韦应贤, 李德越, 等. 4种药剂防治西番莲茎基腐病药效试验初报[J]. 广西植保, 2017(3):36-37.
[14]	卢桂玲, 黄英晴. 百香果根腐病的综合防治技术[J]. 江西农业, 2017(19):23.
[15]	Fischer I H, Rezende J A M. Diseases of passion flower (Passiflora spp.)[J]. Pest Technology, 2008, 2:1-19.
[16]	Bueno C J, Fischer I H, Rosa D D, et al. Fusarium solani f. sp. passiflorae: A new forma specialis causing collar rot in yellow passion fruit[J]. Plant Pathology, 2014, 63(2):382-389. doi: 10.1111/ppa.2014.63.issue-2 URL
[17]	雷娅红, 况卫刚, 郑春生, 等. 基于DNA条形码技术对镰刀菌属的检测鉴定[J]. 植物保护学报, 2016, 43(4):544-551.
[18]	王世伟, 王卿惠, 李小鹏, 等. 镰刀菌分子鉴定与重要应用的研究进展[J]. 微生物学通报, 2018, 45(4):907-919.
[19]	刘树蓬. 内生真菌多样性及其产活性物质研究[D]. 杭州:浙江大学, 2006.
[20]	Rooney-Latham S, Blomquist C L, Scheck H J. First report of Fusarium wilt caused by Fusarium oxysporum f. sp. passiflorae on Passion Fruit in North America[J]. Plant Disease, 2011, 95:1478. doi: 10.1094/PDIS-03-11-0261 pmid: 30731749
[21]	李润根, 曾慧兰, 卢其能, 等. 百合枯萎病菌Fusarium commune的鉴定及其对杀菌剂敏感性研究[J]. 园艺学报, 2020(1):162-172.
[22]	Caroline B M, Martijn R. Pathogen profile update: Fusarium oxysporum[J]. Molecular Plant Pathology, 2009, 10(3):311-324. doi: 10.1111/mpp.2009.10.issue-3 URL
[23]	Munkvold G P. Fusarium species and their associated mycotoxins[J]. Methods in molecular biology, 2017, 1542:51-106. pmid: 27924531
[24]	Sharma L, Marques G. Fusarium, an entomopathogen-a myth or reality?[J]. Pathogens, 2018, 7(4):1-15. doi: 10.3390/pathogens7010001 URL
[25]	曹雪梅, 李生兵, 张惠玲, 等. 甘草根腐病病原菌鉴定[J]. 植物病理学报, 2014, 44(2):213-216.
[26]	林泗海, 黄添基, 傅成业. 西番莲茎基腐病的发生特点及防治技术[J]. 现代园艺, 2015(7):128.
[27]	Khan N, Maymon M, Hirsch Ann M. Combating Fusarium infection using bacillus- based antimicrobials[J]. Microorganisms, 2017, 5(4):1-13. doi: 10.3390/microorganisms5010001 URL
[28]	Silva A S, Oliveira E J, Haddad F, et al. Identification of passion fruit genotypes resistant to Fusarium oxysporum f. sp. passiflorae[J]. Tropical Plant Pathol, 2013, 38(3):236-242. doi: 10.1590/S1982-56762013005000008 URL

编号	采集地	菌落颜色	菌落形态	大型分生孢子	小分生孢子	孢子大小/μm
编号	采集地	菌落颜色	菌落形态	大型分生孢子	小分生孢子	大	小
FP1	广州	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	52.1~165.4	40.2~63.4
FP2	河源	白	气生菌丝薄绒状	镰刀型,两端较钝,2~3个隔	椭圆形	45.3~88.2	25.5~43.3
FP3	清远	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	75.4~185.2	33.2~50.6
FP4	韶关	米白	气生菌丝薄绒状,较紧密	镰刀型,两端较钝,2~4个隔	长卵形	48.1~175.8	44.2~65.7
FP5	肇庆	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	88.1~165.2	45.8~63.8
FP6	四会	白	气生菌丝薄绒状,较紧密	镰刀型,两端较钝,2~4个隔	长卵形	78.1~175.4	36.3~53.3
FP7	四会	浅粉	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	65.7~175.2	45.7~63.1
FP8	茂名	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~3个隔	椭圆形	78.1~185.2	45.8~62.4

编号	采集地	菌落颜色	菌落形态	大型分生孢子	小分生孢子	孢子大小/μm
编号	采集地	菌落颜色	菌落形态	大型分生孢子	小分生孢子	大	小
FP1	广州	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	52.1~165.4	40.2~63.4
FP2	河源	白	气生菌丝薄绒状	镰刀型,两端较钝,2~3个隔	椭圆形	45.3~88.2	25.5~43.3
FP3	清远	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	75.4~185.2	33.2~50.6
FP4	韶关	米白	气生菌丝薄绒状,较紧密	镰刀型,两端较钝,2~4个隔	长卵形	48.1~175.8	44.2~65.7
FP5	肇庆	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	88.1~165.2	45.8~63.8
FP6	四会	白	气生菌丝薄绒状,较紧密	镰刀型,两端较钝,2~4个隔	长卵形	78.1~175.4	36.3~53.3
FP7	四会	浅粉	气生菌丝薄绒状	镰刀型,两端较钝,2~4个隔	长卵形	65.7~175.2	45.7~63.1
FP8	茂名	米白	气生菌丝薄绒状	镰刀型,两端较钝,2~3个隔	椭圆形	78.1~185.2	45.8~62.4

编号	名称	稀释倍数	菌株直径/cm	抑制率/%	病情等级
T0	对照		9.8±0.10a	0.00f	5
T1	99%恶霉灵	4000	7.43±0.10b	25.19±1.06d	5
T2	45%咪鲜胺	1000	1.1±0.05f	95.56±2.06a	3
T3	80%烯酰吗啉	1000	5.86±0.04c	42.59±0.45c	5
T4	33%春雷王铜	1000	4.86±0.03d	53.78±0.98bc	4
T5	30%苯甲嘧菌酯	1500	3.93±0.06e	64.07±2.16b	2
T6	60%霜脲嘧菌酯	1500	4.23±0.06de	60.74±1.11b	3
T7	12%甲密甲霜灵	1500	4.33±0.15de	59.63±2.01b	3
T8	24%噻呋酰胺	1500	8.5±0.10ab	13.33±0.36e	4

编号	名称	稀释倍数	菌株直径/cm	抑制率/%	病情等级
T0	对照		9.8±0.10a	0.00f	5
T1	99%恶霉灵	4000	7.43±0.10b	25.19±1.06d	5
T2	45%咪鲜胺	1000	1.1±0.05f	95.56±2.06a	3
T3	80%烯酰吗啉	1000	5.86±0.04c	42.59±0.45c	5
T4	33%春雷王铜	1000	4.86±0.03d	53.78±0.98bc	4
T5	30%苯甲嘧菌酯	1500	3.93±0.06e	64.07±2.16b	2
T6	60%霜脲嘧菌酯	1500	4.23±0.06de	60.74±1.11b	3
T7	12%甲密甲霜灵	1500	4.33±0.15de	59.63±2.01b	3
T8	24%噻呋酰胺	1500	8.5±0.10ab	13.33±0.36e	4