Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (4): 25-30.doi: 10.11924/j.issn.1000-6850.casb20200300246

Special Issue: 生物技术

Previous Articles     Next Articles

Research Progress of Cell Wall Regeneration from Plant Protoplast

Yang Ying(), Kang Lan, Geng Xin, Chen Yuzhen, Lu Cunfu()   

  1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083
  • Received:2020-03-23 Revised:2020-05-14 Online:2021-02-05 Published:2021-01-25
  • Contact: Lu Cunfu E-mail:yangying1994@bjfu.edu.cn;lucunfu@bjfu.edu.cn

Abstract:

Protoplasts without cell walls are capable of forming new cell walls and therefore provide a unique model and perspective for the study of cell wall biosynthesis. The process of cell wall regeneration from plant protoplast is extremely complex, involving a lot of signal transduction pathway, thus, it is difficult to thoroughly study the molecular network of the whole regeneration wall process by using common research methods. To understand the intrinsic characteristics of the new regenerated cell wall from plant protoplasts, and promote further understanding of the molecular mechanism of protoplast regeneration, three parts were comprehensively discussed in this review, including a detailed elaboration about different types of protoplast culture, the cytobiological evidence of cell wall regeneration and the molecular mechanism of cell wall regeneration combined with key omics techniques. In addition, the main influencing factors of different plant protoplast culture such as the limitation of plant materials, culture medium and culture methods were analyzed. Two kinds of dyes commonly used in the visualization of regenerated cell wall and the transcriptome, proteome and more intricate nuclear proteome and phosphoproteome used in the construction of cell wall molecular networks were summarized. At the same time, the further study of mechanism of cell wall regeneration was prospected.

Key words: protoplast, cell wall regeneration, tissue culture, Calcofluor white, molecular mechanism

CLC Number: