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Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (3): 102-109.doi: 10.11924/j.issn.1000-6850.casb2021-0229

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Optimization of Culture Medium of Serendipita indica Based on Response Surface Methodology

LI Jiahuan1(), WANG Xiaohui1, JIANG Mingguo2, SONG Fuqiang1, CHANG Wei1()   

  1. 1Key Laboratory of Ecological Restoration and Resource Utilization in the Cold Region of Heilongjiang Province, Heilongjiang University, Harbin 150080
    2Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008
  • Received:2021-03-09 Revised:2021-06-13 Online:2022-01-25 Published:2022-02-25
  • Contact: CHANG Wei E-mail:lijiahuan_1998@163.com;changwei77@126.com

Abstract:

The root endophytic fungus Serendipita indica can be cultured on artificial medium. The present study aims at optimizing the medium to enhance the growth rate of S. indica. In this experiment, the single-factor experiment was selected for evaluating the effect of six parameters on the growth rate of S. indica, and the optimization design of the medium was performed by the response surface methodology (RSM). The results showed that the optimum temperature was 31±2℃, the optimum pH was 6.9±0.2, the best carbon source was sucrose, the best nitrogen source was NaNO3, and the best inorganic salt was MgSO4·7H2O. The optimal medium formulation was determined by RSM as following: MgSO4·7H2O 0.824 g/L, KH2PO4 1.118 g/L, NaNO3 1.055 g/L, NaCl 0.03 g/L, CaCl2 0.05 g/L and sucrose 10 g/L. In the optimal medium, the average growth rate of S. indica reached 1.8083 cm/d, which was greatly faster than that in the original MMN medium. In this study, medium optimization based on RSM improved the growth rate of S. indica, which laid a foundation for the large-scale production of S. indica and its application in field trials.

Key words: Serendipita indica, medium, response surface method, single factor optimization, growth rate

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