Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (3): 102-109.doi: 10.11924/j.issn.1000-6850.casb2021-0229
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LI Jiahuan1(), WANG Xiaohui1, JIANG Mingguo2, SONG Fuqiang1, CHANG Wei1()
Received:
2021-03-09
Revised:
2021-06-13
Online:
2022-01-25
Published:
2022-02-25
Contact:
CHANG Wei
E-mail:lijiahuan_1998@163.com;changwei77@126.com
CLC Number:
LI Jiahuan, WANG Xiaohui, JIANG Mingguo, SONG Fuqiang, CHANG Wei. Optimization of Culture Medium of Serendipita indica Based on Response Surface Methodology[J]. Chinese Agricultural Science Bulletin, 2022, 38(3): 102-109.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2021-0229
培养基 | 配方 |
---|---|
PDMA | 葡萄糖20 g;20%马铃薯汁500 mL;麦芽浸粉(波美3度)500 mL;琼脂15 g |
综合PDA | 葡萄糖20 g;20%马铃薯汁1000 mL;VB1(0.05 g);MgSO4·7H2O 1.5 g;KH2PO4 3 g;琼脂15 g |
PDA | 葡萄糖20 g;土豆200 g;水1000 mL;琼脂15 g |
MMN | 葡萄糖10 g;麦芽汁(波美3度)100 mL;VB1 0.1 g;MgSO4·7H2O 0.15 g;KH2PO4 0.5 g;NaCl 0.025 g; CaCl2 0.05 g;(NH4)2HPO4 0.25 g; FeCl3 (1%) 1.2 mL;柠檬酸0.2 g;水900 mL;琼脂15 g |
改良MMN | 葡萄糖10 g;麦芽汁3 g;VB1(0.05 g);MgSO4·7H2O 0.15 g;KH2PO4 0.5 g;NaCl 0.025 g;CaCl2 0.05 g; (NH4)2HPO4 0.25 g;FeCl3(1%) 1.2 mL;牛肉膏15 g;蛋白胨15 g;水1000 mL;琼脂15 g |
KM | 葡萄糖20 g;水解酪蛋白1 g;酵母提取物1 g;蛋白胨2 g;20×salt stock solution 50 mL;100×Microelement stock solution 10 mL;Fe-EDTA10 mL;1000×Vitamin stock solution 1 mL;水930 mL;琼脂15 g;20×salt stock solution:NaNO3 12 g;KCl 10.4 g;MgSO4·7H2O 10.4 g;KH2PO4 30.4 g;水1000 mL;100×Microelement stock solution:ZnSO4·7H2O 2.2 g;H3BO4 1 g;MnSO4·H2O 0.5 g;CoCl·5H2O 0.16 g;CuSO4·5H2O 0.16 g;(NH4)6MO7O24 0.11 g;水1 000 mL;Fe-EDTA:FeSO4 0.13 g;EDTA 0.18 g;水100 mL;1000×Vitamin stock solution:VB1 (0.05 g);VB3 (0.01 g);VB6 (0.01 g);甘氨酸0.04 g;1000 mL |
培养基 | 配方 |
---|---|
PDMA | 葡萄糖20 g;20%马铃薯汁500 mL;麦芽浸粉(波美3度)500 mL;琼脂15 g |
综合PDA | 葡萄糖20 g;20%马铃薯汁1000 mL;VB1(0.05 g);MgSO4·7H2O 1.5 g;KH2PO4 3 g;琼脂15 g |
PDA | 葡萄糖20 g;土豆200 g;水1000 mL;琼脂15 g |
MMN | 葡萄糖10 g;麦芽汁(波美3度)100 mL;VB1 0.1 g;MgSO4·7H2O 0.15 g;KH2PO4 0.5 g;NaCl 0.025 g; CaCl2 0.05 g;(NH4)2HPO4 0.25 g; FeCl3 (1%) 1.2 mL;柠檬酸0.2 g;水900 mL;琼脂15 g |
改良MMN | 葡萄糖10 g;麦芽汁3 g;VB1(0.05 g);MgSO4·7H2O 0.15 g;KH2PO4 0.5 g;NaCl 0.025 g;CaCl2 0.05 g; (NH4)2HPO4 0.25 g;FeCl3(1%) 1.2 mL;牛肉膏15 g;蛋白胨15 g;水1000 mL;琼脂15 g |
KM | 葡萄糖20 g;水解酪蛋白1 g;酵母提取物1 g;蛋白胨2 g;20×salt stock solution 50 mL;100×Microelement stock solution 10 mL;Fe-EDTA10 mL;1000×Vitamin stock solution 1 mL;水930 mL;琼脂15 g;20×salt stock solution:NaNO3 12 g;KCl 10.4 g;MgSO4·7H2O 10.4 g;KH2PO4 30.4 g;水1000 mL;100×Microelement stock solution:ZnSO4·7H2O 2.2 g;H3BO4 1 g;MnSO4·H2O 0.5 g;CoCl·5H2O 0.16 g;CuSO4·5H2O 0.16 g;(NH4)6MO7O24 0.11 g;水1 000 mL;Fe-EDTA:FeSO4 0.13 g;EDTA 0.18 g;水100 mL;1000×Vitamin stock solution:VB1 (0.05 g);VB3 (0.01 g);VB6 (0.01 g);甘氨酸0.04 g;1000 mL |
Run No. | 硫酸镁/(g/L) | 磷酸二 氢钾/(g/L) | 硝酸钠/(g/L) | 菌落生长 速率/(cm/d) |
---|---|---|---|---|
1 | 1.15 | 1.4 | 0.4 | 1.7750 |
2 | 1.15 | 0.4 | 1.4 | 1.7000 |
3 | 0.65 | 1.4 | 1.4 | 1.8500 |
4 | 0.15 | 2.4 | 1.4 | 1.0300 |
5 | 0.15 | 1.4 | 0.4 | 1.3433 |
6 | 1.15 | 1.4 | 2.4 | 1.6333 |
7 | 0.15 | 0.4 | 1.4 | 1.4167 |
8 | 0.65 | 2.4 | 2.4 | 1.4800 |
9 | 0.65 | 0.4 | 0.4 | 1.7750 |
10 | 0.65 | 2.4 | 0.4 | 1.6100 |
11 | 0.15 | 1.4 | 2.4 | 1.2617 |
12 | 0.65 | 0.4 | 2.4 | 1.7250 |
13 | 1.15 | 2.4 | 1.4 | 1.6217 |
14 | 0.65 | 1.4 | 1.4 | 1.8417 |
15 | 0.65 | 1.4 | 1.4 | 1.8500 |
16 | 0.65 | 1.4 | 1.4 | 1.8500 |
17 | 0.65 | 1.4 | 1.4 | 1.8750 |
Run No. | 硫酸镁/(g/L) | 磷酸二 氢钾/(g/L) | 硝酸钠/(g/L) | 菌落生长 速率/(cm/d) |
---|---|---|---|---|
1 | 1.15 | 1.4 | 0.4 | 1.7750 |
2 | 1.15 | 0.4 | 1.4 | 1.7000 |
3 | 0.65 | 1.4 | 1.4 | 1.8500 |
4 | 0.15 | 2.4 | 1.4 | 1.0300 |
5 | 0.15 | 1.4 | 0.4 | 1.3433 |
6 | 1.15 | 1.4 | 2.4 | 1.6333 |
7 | 0.15 | 0.4 | 1.4 | 1.4167 |
8 | 0.65 | 2.4 | 2.4 | 1.4800 |
9 | 0.65 | 0.4 | 0.4 | 1.7750 |
10 | 0.65 | 2.4 | 0.4 | 1.6100 |
11 | 0.15 | 1.4 | 2.4 | 1.2617 |
12 | 0.65 | 0.4 | 2.4 | 1.7250 |
13 | 1.15 | 2.4 | 1.4 | 1.6217 |
14 | 0.65 | 1.4 | 1.4 | 1.8417 |
15 | 0.65 | 1.4 | 1.4 | 1.8500 |
16 | 0.65 | 1.4 | 1.4 | 1.8500 |
17 | 0.65 | 1.4 | 1.4 | 1.8750 |
回归 | 平方和 | 自由度 | 均方 | F值 | P概率 | 显著性 |
---|---|---|---|---|---|---|
模型 | 0.950 | 9 | 0.110 | 390.34 | <0.0001 | ** |
X1 | 0.350 | 1 | 0.350 | 1301.81 | <0.0001 | ** |
X2 | 0.096 | 1 | 0.096 | 353.86 | <0.0001 | ** |
X3 | 0.020 | 1 | 0.020 | 75.17 | <0.0001 | ** |
X12 | 0.320 | 1 | 0.320 | 1200.63 | <0.0001 | ** |
X22 | 0.075 | 1 | 0.075 | 277.59 | <0.0001 | ** |
X32 | 0.022 | 1 | 0.022 | 81.4 | <0.0001 | ** |
X1X2 | 0.024 | 1 | 0.024 | 87.92 | <0.0001 | ** |
X1X3 | 0.000903 | 1 | 0.000903 | 3.34 | 0.1104 | |
X2 X3 | 0.0016 | 1 | 0.0016 | 5.92 | 0.0453 | * |
残差 | 0.001893 | 5 | 0.0002705 | |||
失拟项 | 0.001255 | 3 | 0.0004184 | 2.62 | 0.1873 | 不显著 |
纯误差 | 0.0006381 | 2 | 0.0001595 | |||
总差 | 0.950 | 16 |
回归 | 平方和 | 自由度 | 均方 | F值 | P概率 | 显著性 |
---|---|---|---|---|---|---|
模型 | 0.950 | 9 | 0.110 | 390.34 | <0.0001 | ** |
X1 | 0.350 | 1 | 0.350 | 1301.81 | <0.0001 | ** |
X2 | 0.096 | 1 | 0.096 | 353.86 | <0.0001 | ** |
X3 | 0.020 | 1 | 0.020 | 75.17 | <0.0001 | ** |
X12 | 0.320 | 1 | 0.320 | 1200.63 | <0.0001 | ** |
X22 | 0.075 | 1 | 0.075 | 277.59 | <0.0001 | ** |
X32 | 0.022 | 1 | 0.022 | 81.4 | <0.0001 | ** |
X1X2 | 0.024 | 1 | 0.024 | 87.92 | <0.0001 | ** |
X1X3 | 0.000903 | 1 | 0.000903 | 3.34 | 0.1104 | |
X2 X3 | 0.0016 | 1 | 0.0016 | 5.92 | 0.0453 | * |
残差 | 0.001893 | 5 | 0.0002705 | |||
失拟项 | 0.001255 | 3 | 0.0004184 | 2.62 | 0.1873 | 不显著 |
纯误差 | 0.0006381 | 2 | 0.0001595 | |||
总差 | 0.950 | 16 |
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