Propagation of Arbuscular Mycorrhizal Fungi: A Review

doi:10.11924/j.issn.1000-6850.casb2021-0427

Abstract

Abstract:

In the absence of plant symbiosis, arbuscular mycorrhiza (AM) fungi cannot be purely cultured on medium like other fungi, which greatly restricts the basic and applied research on AM fungi. This study aims at clarifying the development process and characteristics of different culture methods of arbuscular mycorrhiza (AM) fungi, and achieving the goal of pure culture of AM fungi. The authors reviewed and compared the symbiotic culture methods between AM fungi and the whole plant, including pot culture method, nutrient film technique, aeroponics and medium culture. The authors also summarized the dual culture methods between AM fungi and plant roots, including non-transformed root culture and transformed root culture. At last, the authors put forward some future research focuses in order to provide reference for the research of AM fungi culture and symbiotic mechanism.

Key words: arbuscular mycorrhizal fungi, symbiotic culture, dual culture, symbiotic mechanism

CLC Number:

S154.3

LI Shaojie, XIAO Qingshan, SONG Fuqiang, WANG Xin. Propagation of Arbuscular Mycorrhizal Fungi: A Review[J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 115-122.

Figures/Tables 2

References 56

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AM真菌种类	繁殖体	宿主根	培养基	培养方式	参考文献
Glomus intraradicesMUCL 49410	孢子	Medicago truncatula	MSR	单室培养	[12]
Glomus intraradicesDAOM 197198	孢子	Lycopersicon esculentum wild-type 76R and rmc	M	单室培养	[13]
Glomus intraradicesMUCL 43204	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus intraradices	孢子	Solanum lycopersicum L.	M	单室培养	[15]
Gigaspora margarita	孢子	Lycopersicon esculentum Mill.	MS	单室培养	[16]
Gigaspora margarita	孢子	Linum usitatissimim L. Tagetes pastula L. Althaea offificinalis L.	M	单室培养	[17]
Glomus proliferumMUCL 41827	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus mosseae	孢子	Lycopersicum esculentum Mill. Trifolium pratense L.	White	单室培养	[11]
Glomus mosseae	孢子	Saccharum offificinarum Sorghum vulgare	M	单室培养	[18]
Glomus versiforme	囊泡	Solanum lycipersicum L.	M	单室培养	[19]

AM真菌种类	繁殖体	宿主根	培养基	培养方式	参考文献
Glomus intraradicesMUCL 49410	孢子	Medicago truncatula	MSR	单室培养	[12]
Glomus intraradicesDAOM 197198	孢子	Lycopersicon esculentum wild-type 76R and rmc	M	单室培养	[13]
Glomus intraradicesMUCL 43204	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus intraradices	孢子	Solanum lycopersicum L.	M	单室培养	[15]
Gigaspora margarita	孢子	Lycopersicon esculentum Mill.	MS	单室培养	[16]
Gigaspora margarita	孢子	Linum usitatissimim L. Tagetes pastula L. Althaea offificinalis L.	M	单室培养	[17]
Glomus proliferumMUCL 41827	孢子	Lunularia cruciata	SRV	单室培养	[14]
Glomus mosseae	孢子	Lycopersicum esculentum Mill. Trifolium pratense L.	White	单室培养	[11]
Glomus mosseae	孢子	Saccharum offificinarum Sorghum vulgare	M	单室培养	[18]
Glomus versiforme	囊泡	Solanum lycipersicum L.	M	单室培养	[19]

丛枝菌根真菌种类	繁殖体	发根农杆菌种类	宿主根	培养基	培养方式	参考文献
Glomus intraradices CMCCROC7	孢子	Ri1600	Solanum tuberosum var ‘Pukhraj'	M	单室培养	[23]
Glomus intraradices MUCL43194	孢子	K599	Astragalua sinicus L.	MSR	单室培养	[24]
Glomus intraradices	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	UNSW	Daucus carota L.	M	单室培养	[26]
	孢子	A4	Lycopersicon esculentum Micro-Tom	M	单室培养	[27]
	孢子	---	Daucus carota L.	M	单室培养	[15]
	孢子	---	Daucus carota L.	M	双室培养	[28]
	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
	孢子	A4T	Medicago truncatula Gaertn.	M	单室培养	[30]
	囊泡	---	Daucus carota L.	M	双室培养	[31]
Rhizophagus intraradices (GC3)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]
Rhizophagus irregularis DAOM 181602	孢子	A4	Daucus carota L.	M	单室培养	[33]
Rhizophagus irregularis C3、DAOM 197198	孢子	A4	Daucus carota L.	M、MS	双室培养	[34]
Rhizophagus intraradices	孢子	LBA9402	Lotus japonicus	MSR	单室培养	[35]
	孢子	---	Ocimum basilicum	M	单室培养	[36]
Gigaspora margarita	孢子	A4、LBA9402、ATCC15834	Lycopersicon esculantum L.var. Pusa Ruby.	MS、M	单室培养	[37]
	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
Gigaspora decipiens (JA2 strain)	孢子	A4	Daucus carota L.	M	单室培养	[39]
Gigaspora decipiens	孢子	A4	Daucus carota L.	M	单室培养	[40]
Glomus clarum	孢子	A4	Daucus carota L.	M	单室培养	[40]
	孢子	LBA9402	Daucus carota L. Trifolium repens L.	M	单室培养	[41]
Glomus proliferum	囊泡	---	Daucus carota L.	MSR	单室培养双室培养	[42]
Gigaspora gigantea	孢子	A4、R1000	Solanum lycopersicum L.	MSR	单室培养	[43]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
	孢子	----	Daucus carota L.	G+T	单室培养	[44]
Funneliformis mosseae	孢子	K599	Daucus carota L.	MSR	单室培养	[45]
Gigaspora roseae	孢子	A4	Daucus carota L.	M	单室培养	[46]
Scutellospora reticulata	孢子	----	Daucus carota L.	MSR	单室培养	[47]
Acaulospora rehmii	孢子	---	Daucus carota L.	MSR	单室培养	[48]
Glomus caledonium	孢子	----	Daucus carota L.	M	单室培养	[49]
Glomus etunicatum	孢子	A4	Daucus carota L.	M	单室培养	[50]
Glomus fasciculatum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus fistulosum	孢子	A4	Fragaria x Ananassa Duchesne.	M	单室培养	[51]
Glomus mosseae	孢子	---	Daucus carota L. Convolvulus sepium L. Solanum tuberosum L. Vigna unguiculata Walp.		双室培养	[21]
	孢子+囊泡	---	Daucus carota L.	M	单室培养	[17]
	孢子	---	Daucus carota L.	M	单室培养	[52]
Glomus macrocarpum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus versiforme	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Rhizophagus aggregatus (GX4)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]

丛枝菌根真菌种类	繁殖体	发根农杆菌种类	宿主根	培养基	培养方式	参考文献
Glomus intraradices CMCCROC7	孢子	Ri1600	Solanum tuberosum var ‘Pukhraj'	M	单室培养	[23]
Glomus intraradices MUCL43194	孢子	K599	Astragalua sinicus L.	MSR	单室培养	[24]
Glomus intraradices	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	UNSW	Daucus carota L.	M	单室培养	[26]
	孢子	A4	Lycopersicon esculentum Micro-Tom	M	单室培养	[27]
	孢子	---	Daucus carota L.	M	单室培养	[15]
	孢子	---	Daucus carota L.	M	双室培养	[28]
	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
	孢子	A4T	Medicago truncatula Gaertn.	M	单室培养	[30]
	囊泡	---	Daucus carota L.	M	双室培养	[31]
Rhizophagus intraradices (GC3)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]
Rhizophagus irregularis DAOM 181602	孢子	A4	Daucus carota L.	M	单室培养	[33]
Rhizophagus irregularis C3、DAOM 197198	孢子	A4	Daucus carota L.	M、MS	双室培养	[34]
Rhizophagus intraradices	孢子	LBA9402	Lotus japonicus	MSR	单室培养	[35]
	孢子	---	Ocimum basilicum	M	单室培养	[36]
Gigaspora margarita	孢子	A4、LBA9402、ATCC15834	Lycopersicon esculantum L.var. Pusa Ruby.	MS、M	单室培养	[37]
	孢子	clone Gp	Daucus carota L.	M	单室培养	[25]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
Gigaspora decipiens (JA2 strain)	孢子	A4	Daucus carota L.	M	单室培养	[39]
Gigaspora decipiens	孢子	A4	Daucus carota L.	M	单室培养	[40]
Glomus clarum	孢子	A4	Daucus carota L.	M	单室培养	[40]
	孢子	LBA9402	Daucus carota L. Trifolium repens L.	M	单室培养	[41]
Glomus proliferum	囊泡	---	Daucus carota L.	MSR	单室培养双室培养	[42]
Gigaspora gigantea	孢子	A4、R1000	Solanum lycopersicum L.	MSR	单室培养	[43]
	孢子	A4	Daucus carota L.	M	单室培养	[38]
	孢子	----	Daucus carota L.	G+T	单室培养	[44]
Funneliformis mosseae	孢子	K599	Daucus carota L.	MSR	单室培养	[45]
Gigaspora roseae	孢子	A4	Daucus carota L.	M	单室培养	[46]
Scutellospora reticulata	孢子	----	Daucus carota L.	MSR	单室培养	[47]
Acaulospora rehmii	孢子	---	Daucus carota L.	MSR	单室培养	[48]
Glomus caledonium	孢子	----	Daucus carota L.	M	单室培养	[49]
Glomus etunicatum	孢子	A4	Daucus carota L.	M	单室培养	[50]
Glomus fasciculatum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus fistulosum	孢子	A4	Fragaria x Ananassa Duchesne.	M	单室培养	[51]
Glomus mosseae	孢子	---	Daucus carota L. Convolvulus sepium L. Solanum tuberosum L. Vigna unguiculata Walp.		双室培养	[21]
	孢子+囊泡	---	Daucus carota L.	M	单室培养	[17]
	孢子	---	Daucus carota L.	M	单室培养	[52]
Glomus macrocarpum	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Glomus versiforme	囊泡	---	Daucus carota L.	MSR	单室培养	[29]
Rhizophagus aggregatus (GX4)	孢子	A4	Daucus carota L.	M+土壤	双室培养	[32]