Abstract:

To optimize the extraction procedure of atractylodin, atractylone and β-Eudesmol of Atractylodes chinensis (DC.) Koidz and the HPLC analytical method, the sample consumption, ultrasonic time and ultrasonic temperature in the procedure of sample extraction were analyzed, and chromatographic conditions were investigated one by one. The extraction condition was as follows: sample consumption of 0.8 g, and ultrasonication at 40℃ for 60 min. The chromatographic condition was as follows: chromatographic column (Venusil MP C18, 4.6×150 mm, 5 μm, 150 A), and column temperature of 28℃. To detect atractylodin and atractylone, methanol: water (82:18) was used as the mobile phase; to detect β-Eudesmol, acetonitrile: 0.2% sodium bicarbonate: 10% methanol (65:25:10) was used as the mobile phase. In the range of 0.002-1 mg/mL, the peak areas of atractylodin, atractylone and β-Eudesmol had good linear relationships (R²=0.9999-1, n=6). The average recovery ratio was 97.7%-102.17%, RSD was 1.52%-2.49%, n=6. This method is accurate and simple and it could be used for determining the content of atractylodin, atractylone and β-Eudesmol of A.chinensis of 1-4 years old.

Key words: Atractylodes chinensis (DC.) Koidz, HPLC, atractylodin, atractylone, β-Eudesmol