Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (2): 48-52.doi: 10.11924/j.issn.1000-6850.casb18100033

Previous Articles     Next Articles

Tamarix chinensis: Technique of Tissue Culture and Rapid Propagation

Wei Haixia, Wang Xia, Li Shuangyun, Zhou Jian, Wang Lili, Liu Dexi()   

  1. Shandong Academy of Forestry Sciences, Jinan 250014
  • Received:2018-10-11 Revised:2019-05-21 Online:2020-01-15 Published:2020-04-30
  • Contact: Liu Dexi E-mail:llyldx@163.com

Abstract:

The paper aims to better preserve the germplasm of Tamarix chinensis. We took the stems of Tamarix chinensis segments as explants and carried out the tissue culture and rapid propagation. The results show that the optimum sterilization scheme is: soaked in 75% ethanol for 30 s, 2% sodium hypochlorite for 3-5 min and mercury dichloride for 5 min. MS+sucrose 20 g/L+agar 6.5 g/L is the optimum primary medium for Tamarix chinensis, and MS+sucrose 30 g/L+agar 7 g/L+6-BA 0.5 mg/L+NAA 0.1 mg/L is the optimum subculture medium for Tamarix chinensis, 1/2MS medium is the best rooting medium.

Key words: Tamarix chinensis, rapid propagation, multiplication, rooting, medium

CLC Number: