Effects of Supplemental Light Quality and Duration on the Growth and Carbon Metabolism of Leaves of Greenhouse-grown Sweet Pepper

doi:10.11924/j.issn.1000-6850.casb2022-0626

Abstract

Abstract:

To explore the effect of LED (light emitting diode) supplemental light on the growth and carbon metabolism of leaves of greenhouse-grown sweet pepper (Capsium annuum), ‘Aodaili’ was chosen as the material to investigate the effects of different light qualities and duration on the growth, mineral elements’ content, sugar content and activities of key carbon metabolic enzymes. The LED as light source was provided with three light qualities, including the ratio of red (R) and blue (B) light of 2:1(2R1B), 4:1(4R1B) and 8:1(8R1B), and three supplemental light duration, including 2 h (18:00-20:00), 4 h (18:00-22:00) and 8 h (18:00-02:00 next day), and no supplemental light was taken as the control (CK). The results showed that the growth and carbon metabolism of leaves of sweet pepper were significantly affected by light quality and light duration, which had an interaction between them. Compared with those of CK, the specific leaf weight (SLW), the contents of potassium, fructose and starch significantly decreased under 2R1B treatment; while the leaf number, total leaf area, total chlorophyll content, biomass net assimilation rate (NAR), total sugar content, and the activities of acid invertase (AI), neutral invertase (NI) and sucrose synthase (SS) were significantly higher than those of CK under 4R1B or 8R1B. Moreover, the 8R1B treatment was slightly better than 4R1B treatment, indicating that increasing the proportion of red light was beneficial to leaf growth and carbon metabolism. Light quality had no significant effect on the contents of nitrogen, phosphorus, potassium and magnesium in leaves of sweet pepper. Under the same light quality, the leaf number, SLW, leaf area index (LAI), NAR, total sugar content, fructose content, starch content and the activities of AI, NI, SS and sucrose phosphate synthase (SPS) of sweet pepper increased gradually with the duration of supplemental light except the 2R1B treatment. Moreover, these indexes were significantly higher under light treatment of 8 h than those under 2 h. In conclusion, increasing the proportion of red light and the duration of supplemental light is beneficial to the leaf growth, and can improve carbon metabolism and sugar content, and increase dry matter accumulation. The treatment of 8 h under 8R1B is optimal for sweet pepper.

Key words: sweet pepper, light quality, supplemental light duration, growth, carbon metabolism

CLC Number:

S641.3

DUAN Qingqing, HAN Meimei, TAN Yueqiang, ZHANG Zikun. Effects of Supplemental Light Quality and Duration on the Growth and Carbon Metabolism of Leaves of Greenhouse-grown Sweet Pepper[J]. Chinese Agricultural Science Bulletin, 2023, 39(1): 37-44.

Figures/Tables 6

References 38

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处理		叶片数	总叶面积/ (m²/株)	比叶重/ (mg/cm²)	叶面积指数	叶绿素总量/ [mg/(g·FW)]	生物量净积累速率/ [g/(m²·d)]
光质	补光时间/h	叶片数	总叶面积/ (m²/株)	比叶重/ (mg/cm²)	叶面积指数	叶绿素总量/ [mg/(g·FW)]	生物量净积累速率/ [g/(m²·d)]
2R1B	2	105.5±5.44bc	0.29±0.03b	8.01±0.11cd	1.12±0.05c	2.28±0.16c	0.64±0.03c
	4	108.0±6.14bc	0.31±0.04ab	8.04±0.10cd	1.26±0.06bc	2.37±0.13c	0.75±0.05b
	8	81.5±4.37d	0.23±0.01c	7.43±0.21d	1.15±0.10c	2.30±0.11c	0.72±0.03b
4R1B	2	120.4±7.49ab	0.33±0.02a	8.51±0.26bc	1.36±0.11b	3.12±0.21b	0.73±0.01b
	4	118.6±5.36ab	0.31±0.01ab	8.72±0.22b	1.27±0.19bc	3.01±0.16b	0.76±0.02b
	8	123.0±6.60a	0.39±0.02a	8.96±0.31b	1.75±0.08a	3.42±0.20ab	0.93±0.08a
8R1B	2	120.2±5.68ab	0.35±0.01a	9.11±0.37ab	1.57±0.02b	3.54±0.19a	0.75±0.01b
	4	121.3±4.60a	0.33±0.04a	9.25±0.21a	1.39±0.12b	3.13±0.18b	0.93±0.07a
	8	125.5±5.39a	0.38±0.02a	9.70±0.36a	1.79±0.10a	3.38±0.22ab	1.04±0.06a
CK		94.8±4.14c	0.25±0.03bc	8.18±0.15c	1.19±0.08c	2.08±0.15c	0.42±0.01d
Two-way ANOVA	光质	*	*	*	*	*	*
	补光时间	ns	ns	ns	*	ns	*
	光质×补光时间	*	*	*	*	*	*

处理		叶片数	总叶面积/ (m²/株)	比叶重/ (mg/cm²)	叶面积指数	叶绿素总量/ [mg/(g·FW)]	生物量净积累速率/ [g/(m²·d)]
光质	补光时间/h	叶片数	总叶面积/ (m²/株)	比叶重/ (mg/cm²)	叶面积指数	叶绿素总量/ [mg/(g·FW)]	生物量净积累速率/ [g/(m²·d)]
2R1B	2	105.5±5.44bc	0.29±0.03b	8.01±0.11cd	1.12±0.05c	2.28±0.16c	0.64±0.03c
	4	108.0±6.14bc	0.31±0.04ab	8.04±0.10cd	1.26±0.06bc	2.37±0.13c	0.75±0.05b
	8	81.5±4.37d	0.23±0.01c	7.43±0.21d	1.15±0.10c	2.30±0.11c	0.72±0.03b
4R1B	2	120.4±7.49ab	0.33±0.02a	8.51±0.26bc	1.36±0.11b	3.12±0.21b	0.73±0.01b
	4	118.6±5.36ab	0.31±0.01ab	8.72±0.22b	1.27±0.19bc	3.01±0.16b	0.76±0.02b
	8	123.0±6.60a	0.39±0.02a	8.96±0.31b	1.75±0.08a	3.42±0.20ab	0.93±0.08a
8R1B	2	120.2±5.68ab	0.35±0.01a	9.11±0.37ab	1.57±0.02b	3.54±0.19a	0.75±0.01b
	4	121.3±4.60a	0.33±0.04a	9.25±0.21a	1.39±0.12b	3.13±0.18b	0.93±0.07a
	8	125.5±5.39a	0.38±0.02a	9.70±0.36a	1.79±0.10a	3.38±0.22ab	1.04±0.06a
CK		94.8±4.14c	0.25±0.03bc	8.18±0.15c	1.19±0.08c	2.08±0.15c	0.42±0.01d
Two-way ANOVA	光质	*	*	*	*	*	*
	补光时间	ns	ns	ns	*	ns	*
	光质×补光时间	*	*	*	*	*	*

处理		氮含量(DW)/%	磷含量(DW)/%	钾含量(DW)/%	钙含量(DW)/%	镁含量(DW)/%
光质	补光时间/h	氮含量(DW)/%	磷含量(DW)/%	钾含量(DW)/%	钙含量(DW)/%	镁含量(DW)/%
2R1B	2	3.22±0.14a	0.32±0.03a	4.17±0.19ab	4.05±0.15ab	0.80±0.07ab
	4	3.43±0.11a	0.31±0.04a	3.69±0.10c	4.45±0.26a	0.76±0.01b
	8	3.44±0.15a	0.28±0.01a	3.83±0.12bc	3.92±0.10b	0.87±0.04a
4R1B	2	3.64±0.19a	0.34±0.03a	3.85±0.06bc	4.04±0.17ab	0.72±0.02b
	4	3.46±0.12a	0.32±0.01a	3.87±0.12bc	4.47±0.29a	0.84±0.06a
	8	3.57±0.10a	0.34±0.02a	3.97±0.13b	4.05±0.18ab	0.72±0.03b
8R1B	2	3.59±0.18a	0.33±0.01a	3.97±0.17b	4.30±0.12a	0.65±0.01c
	4	3.79±0.11a	0.34±0.02a	4.13±0.10ab	4.27±0.19a	0.84±0.06a
	8	3.34±0.19a	0.30±0.02a	2.86±0.14d	1.38±0.07c	0.91±0.05a
CK		3.48±0.13a	0.31±0.03a	4.65±0.12a	4.34±0.28a	0.77±0.02b
Two-way ANOVA	光质	ns	ns	ns	*	ns
	补光时间	ns	ns	*	*	*
	光质×补光时间	ns	ns	*	*	*

处理		氮含量(DW)/%	磷含量(DW)/%	钾含量(DW)/%	钙含量(DW)/%	镁含量(DW)/%
光质	补光时间/h	氮含量(DW)/%	磷含量(DW)/%	钾含量(DW)/%	钙含量(DW)/%	镁含量(DW)/%
2R1B	2	3.22±0.14a	0.32±0.03a	4.17±0.19ab	4.05±0.15ab	0.80±0.07ab
	4	3.43±0.11a	0.31±0.04a	3.69±0.10c	4.45±0.26a	0.76±0.01b
	8	3.44±0.15a	0.28±0.01a	3.83±0.12bc	3.92±0.10b	0.87±0.04a
4R1B	2	3.64±0.19a	0.34±0.03a	3.85±0.06bc	4.04±0.17ab	0.72±0.02b
	4	3.46±0.12a	0.32±0.01a	3.87±0.12bc	4.47±0.29a	0.84±0.06a
	8	3.57±0.10a	0.34±0.02a	3.97±0.13b	4.05±0.18ab	0.72±0.03b
8R1B	2	3.59±0.18a	0.33±0.01a	3.97±0.17b	4.30±0.12a	0.65±0.01c
	4	3.79±0.11a	0.34±0.02a	4.13±0.10ab	4.27±0.19a	0.84±0.06a
	8	3.34±0.19a	0.30±0.02a	2.86±0.14d	1.38±0.07c	0.91±0.05a
CK		3.48±0.13a	0.31±0.03a	4.65±0.12a	4.34±0.28a	0.77±0.02b
Two-way ANOVA	光质	ns	ns	ns	*	ns
	补光时间	ns	ns	*	*	*
	光质×补光时间	ns	ns	*	*	*

处理		总糖/[mg/(g·DW)]	果糖/[mg/(g·DW)]	蔗糖/[mg/(g·DW)]	淀粉/[mg/(g·DW)]
光质	补光时间/h	总糖/[mg/(g·DW)]	果糖/[mg/(g·DW)]	蔗糖/[mg/(g·DW)]	淀粉/[mg/(g·DW)]
2R1B	2	107.15±1.02d	10.16±0.58d	9.21±0.79c	48.82±0.76d
	4	110.68±1.30cd	11.21±0.83cd	9.44±0.94c	50.28±0.95d
	8	96.35±0.98e	10.93±0.91d	10.95±0.81b	40.25±1.14e
4R1B	2	115.44±1.87c	13.76±1.01b	10.65±0.92b	55.46±1.63c
	4	128.06±1.01b	14.16±0.95b	10.87±1.01b	60.17±0.57b
	8	132.82±1.96a	16.68±1.03a	11.09±0.83b	70.05±1.04a
8R1B	2	120.97±1.24b	12.55±1.00c	11.36±0.79ab	58.27±0.66bc
	4	118.53±1.11b	13.39±0.68bc	12.32±0.87a	63.23±0.98b
	8	136.95±0.99a	16.97±1.04a	12.77±1.06a	71.29±0.86a
CK		108.08±0.72d	12.37±0.97c	10.08±0.87bc	53.19±1.90c
Two-way ANOVA	光质	*	*	*	*
	补光时间	*	*	*	*
	光质×补光时间	*	*	*	*