Effects of Different Plant Growth Regulators on Photosynthetic Characteristics of Tea at Low Temperature Stress

doi:10.11924/j.issn.1000-6850.casb2023-0082

Abstract

Abstract:

To investigate the effects of different concentrations of plant growth regulators on the photosynthetic characteristics of tea under low temperature conditions, ‘Taoyuan Big Leaf’ (TY) and ‘Storage Leaf Qi’ (CY) were used as experimental materials to explore the effects of spraying different plant growth regulators under low temperature conditions on the light response curve, leaf net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO₂ concentration (Ci), transpiration rate (Tr), saturated vapor pressure (VpdL), leaf instantaneous water use efficiency (WUE), and stomatal limit value (LS) of tea trees. The experimental results showed that the five growth regulators improved the cold resistance of ‘Taoyuan Big Leaf’ and 'Storage Leaf Qi' to varying degrees. Among them, the TY treatment group had the effects ranking of C₂>D₃>D₁>C₃>E₂>A₁>E₁>B₂>B₁>C₁>A₂>D₂>B₃>A₃>E₃>CK when spraying plant growth regulators on tea at low temperatures, and C₂ and D₃ had the highest comprehensive ranking, with 0.875 and 0.833 respectively, the cumulative contribution rate of the three principal components is 93.931%. The ranking of the effects of spraying plant growth regulators on tea in the CY treatment group at low temperatures was C₂>A₂>D₁>B₂>B₁>E₃>A₁>B₃>D₂>C₃>C₁>E₁>E₂>D₃>A₃>CK, and C₂ and A₂ were ranking higher, with 0.753 and 0.713 respectively, and the cumulative contribution rate of the three principal components is 89.108%. Spraying regulators at low temperatures can increase Pn, Gs, Ci, Tr, and reduce LS, laying the foundation for improving the cold resistance of tea.

Key words: ‘Taoyuan Big Leaf’, ‘Storage leaf Qi’, growth regulator, low temperature stress, photosynthetic characteristics

XIAO Huangqiao, ZHOU Hongcan, JIE Yucheng. Effects of Different Plant Growth Regulators on Photosynthetic Characteristics of Tea at Low Temperature Stress[J]. Chinese Agricultural Science Bulletin, 2024, 40(2): 25-33.

Figures/Tables 12

References 25

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代号	曲线方程	相关系数	最大净光合速率	光饱和点	光补偿点
TY	y=0.0103x³-0.321x²+3.2226x-3.9992	R²=0.9673	6.36	500	14.86
CY	y=0.0108x³-0.309x²+2.8425x-3.4644	R²=0.9694	4.92	350	13.59

代号	曲线方程	相关系数	最大净光合速率	光饱和点	光补偿点
TY	y=0.0103x³-0.321x²+3.2226x-3.9992	R²=0.9673	6.36	500	14.86
CY	y=0.0108x³-0.309x²+2.8425x-3.4644	R²=0.9694	4.92	350	13.59

组别	处理	Pn/[µmol/(m²·s)]	Gs/[mol/(m²·s)]	Ci/[µmol/mol]	Tr/[mmol/(m²·s)]	VpdL/kPa	WUE/[µmol/mmol]	LS
TY	A₁	5.88±0.19^cde	0.33±0.018^def	430.33±9.5^a	1.12±0.055^c	0.37±0.011^d	5.27±0.12^cd	0±0^g
	A₂	5.78±0.21^def	0.36±0.024^abcd	423±8^ab	1.2±0.03^a	0.36±0.008^def	4.82±0.11^ef	0.02±0.01^g
	A₃	5.13±0.18^g	0.34±0.03^bcdef	412.33±11.24^b	1.13±0.031^c	0.36±0.02^def	4.56±0.17^fgh	0.04±0.02^efg
	B₁	6.23±0.32^bc	0.37±0.021^abc	394.67±10.02^c	1.17±0.025^abc	0.35±0.016^ef	5.34±0.2^cd	0.08±0.031^def
	B₂	6.36±0.19^ab	0.38±0.018^a	393.33±7.64^cd	1.19±0.031^ab	0.34±0.015^f	5.36±0.08^cd	0.09±0.035^de
	B₃	5.59±0.29^ef	0.35±0.023^abcde	387.67±8.5^cde	1.15±0.04^bc	0.36±0.013^def	4.88±0.24^ef	0.1±0.05^de
	C₁	6.31±0.29^b	0.33±0.023^def	385±8^cde	1.13±0.031^c	0.37±0.011^de	5.57±0.4^c	0.11±0.04^cd
	C₂	6.78±0.21^a	0.37±0.022^ab	378±11^de	1.01±0.035^de	0.4±0.011^c	6.7±0.23^a	0.12±0.046^bcd
	C₃	5.38±0.37^fg	0.34±0.023^abcdef	374.33±9.5^e	0.96±0.015^ef	0.42±0.01^b	5.6±0.34^c	0.13±0.047^bcd
	D₁	5.84±0.14^cde	0.33±0.023^cdef	356±10.54^f	0.96±0.02^f	0.43±0.008^ab	6.07±0.1^b	0.17±0.04^bc
	D₂	4.67±0.22^h	0.34±0.021^bcdef	354.33±8.08^f	1.01±0.04^de	0.44±0.01^a	4.62±0.37^efg	0.18±0.046^b
	D₃	5.83±0.21^cde	0.35±0.024^abcde	374.67±8.5^e	1.05±0.025^d	0.45±0.011a	5.54±0.06^c	0.13±0.04^bcd
	E₁	6.18±0.23^bcd	0.36±0.021^abcd	389.33±11.02^cde	1.13±0.031^c	0.36±0.009^def	5.45±0.11^c	0.1±0.056^de
	E₂	5.55±0.31^efg	0.32±0.021^ef	430±10^a	1.12±0.025^c	0.4±0.008^c	4.97±0.24^de	0±0^g
	E₃	4.5±0.32^h	0.31±0.015^f	423±12^ab	1.05±0.02^d	0.36±0.011^def	4.29±0.29^gh	0.02±0.015^fg
	CK	3.18±0.29ⁱ	0.2±0.008^g	297±9^g	0.76±0.031^g	0.3±0.011^g	4.2±0.3^h	0.31±0.05^a
CY	A₁	4.66±0.21^e	0.27±0.012^abc	395±11.53^c	0.31±0.01ⁱ	0.5±0.018^a	15.13±0.19^e	0.08±0.036^ef
	A₂	5.84±0.15^ab	0.27±0.012^abcd	393.33±11.24^c	0.68±0.04^d	0.5±0.017^ab	8.6±0.38^h	0.09±0.035^ef
	A₃	5.32±0.29^cd	0.23±0.023^ef	388±9^c	0.27±0.011^j	0.5±0.012^a	19.91±0.64^a	0.1±0.035^e
	B₁	4.67±0.22^e	0.29±0.018^ab	438±11.14^a	0.57±0.015^f	0.5±0.012^a	8.13±0.18^hi	-0.02±0.012^g
	B₂	5.87±0.19^a	0.28±0.018^ab	417.33±11.06^b	0.3±0.013ⁱ	0.51±0.011^a	19.33±0.42^b	0.03±0.01^fg
	B₃	5.18±0.29^d	0.22±0.016^f	368±10.54^d	0.71±0.013^c	0.5±0.014^a	7.31±0.28^j	0.14±0.03^1e
	C₁	5.33±0.15^cd	0.26±0.012^bcd	294.33±8.5^fgh	0.3±0.012ⁱ	0.48±0.011^b	17.53±0.64^c	0.32±0.035^bcd
	C₂	5.95±0.1^a	0.29±0.017^a	277.33±10.5^hi	0.71±0.013^c	0.5±0.014^ab	8.34±0.16^h	0.36±0.035^b
	C₃	4.67±0.18^e	0.28±0.014^abc	274.33±9.5ⁱ	0.32±0.015ⁱ	0.49±0.013^ab	14.46±0.09^f	0.36±0.06^b
	D₁	5.55±0.18^bc	0.27±0.012^abc	288±9^ghi	0.94±0.009^a	0.48±0.012^b	5.91±0.14^k	0.33±0.05^bc
	D₂	5.75±0.19^ab	0.26±0.013^bcd	289.33±11.02^ghi	0.36±0.011^h	0.43±0.013^cd	15.8±0.47^d	0.33±0.045^bc
	D₃	5.11±0.18^d	0.26±0.018^cde	291.33±10.26^fghi	0.61±0.01^e	0.41±0.011^d	8.33±0.25^h	0.32±0.035^bcd
	E₁	3.96±0.13^f	0.27±0.014^abc	301±10.54^efg	0.92±0.012^a	0.43±0.011^c	4.28±0.09^l	0.3±0.045^bcd
	E₂	5.23±0.13^cd	0.26±0.018^bcd	308±9.54^ef	0.54±0.01^g	0.41±0.011^d	9.72±0.07^g	0.28±0.035^cd
	E₃	5.83±0.21^ab	0.24±0.022^def	316±11.53^e	0.76±0.01^b	0.43±0.011^cd	7.63±0.23^ij	0.26±0.046^d
	CK	2.96±0.19^g	0.18±0.016^g	224.33±12.06^j	0.36±0.013^h	0.31±0.012^e	8.13±0.23^hi	0.48±0.046^a

组别	处理	Pn/[µmol/(m²·s)]	Gs/[mol/(m²·s)]	Ci/[µmol/mol]	Tr/[mmol/(m²·s)]	VpdL/kPa	WUE/[µmol/mmol]	LS
TY	A₁	5.88±0.19^cde	0.33±0.018^def	430.33±9.5^a	1.12±0.055^c	0.37±0.011^d	5.27±0.12^cd	0±0^g
	A₂	5.78±0.21^def	0.36±0.024^abcd	423±8^ab	1.2±0.03^a	0.36±0.008^def	4.82±0.11^ef	0.02±0.01^g
	A₃	5.13±0.18^g	0.34±0.03^bcdef	412.33±11.24^b	1.13±0.031^c	0.36±0.02^def	4.56±0.17^fgh	0.04±0.02^efg
	B₁	6.23±0.32^bc	0.37±0.021^abc	394.67±10.02^c	1.17±0.025^abc	0.35±0.016^ef	5.34±0.2^cd	0.08±0.031^def
	B₂	6.36±0.19^ab	0.38±0.018^a	393.33±7.64^cd	1.19±0.031^ab	0.34±0.015^f	5.36±0.08^cd	0.09±0.035^de
	B₃	5.59±0.29^ef	0.35±0.023^abcde	387.67±8.5^cde	1.15±0.04^bc	0.36±0.013^def	4.88±0.24^ef	0.1±0.05^de
	C₁	6.31±0.29^b	0.33±0.023^def	385±8^cde	1.13±0.031^c	0.37±0.011^de	5.57±0.4^c	0.11±0.04^cd
	C₂	6.78±0.21^a	0.37±0.022^ab	378±11^de	1.01±0.035^de	0.4±0.011^c	6.7±0.23^a	0.12±0.046^bcd
	C₃	5.38±0.37^fg	0.34±0.023^abcdef	374.33±9.5^e	0.96±0.015^ef	0.42±0.01^b	5.6±0.34^c	0.13±0.047^bcd
	D₁	5.84±0.14^cde	0.33±0.023^cdef	356±10.54^f	0.96±0.02^f	0.43±0.008^ab	6.07±0.1^b	0.17±0.04^bc
	D₂	4.67±0.22^h	0.34±0.021^bcdef	354.33±8.08^f	1.01±0.04^de	0.44±0.01^a	4.62±0.37^efg	0.18±0.046^b
	D₃	5.83±0.21^cde	0.35±0.024^abcde	374.67±8.5^e	1.05±0.025^d	0.45±0.011a	5.54±0.06^c	0.13±0.04^bcd
	E₁	6.18±0.23^bcd	0.36±0.021^abcd	389.33±11.02^cde	1.13±0.031^c	0.36±0.009^def	5.45±0.11^c	0.1±0.056^de
	E₂	5.55±0.31^efg	0.32±0.021^ef	430±10^a	1.12±0.025^c	0.4±0.008^c	4.97±0.24^de	0±0^g
	E₃	4.5±0.32^h	0.31±0.015^f	423±12^ab	1.05±0.02^d	0.36±0.011^def	4.29±0.29^gh	0.02±0.015^fg
	CK	3.18±0.29ⁱ	0.2±0.008^g	297±9^g	0.76±0.031^g	0.3±0.011^g	4.2±0.3^h	0.31±0.05^a
CY	A₁	4.66±0.21^e	0.27±0.012^abc	395±11.53^c	0.31±0.01ⁱ	0.5±0.018^a	15.13±0.19^e	0.08±0.036^ef
	A₂	5.84±0.15^ab	0.27±0.012^abcd	393.33±11.24^c	0.68±0.04^d	0.5±0.017^ab	8.6±0.38^h	0.09±0.035^ef
	A₃	5.32±0.29^cd	0.23±0.023^ef	388±9^c	0.27±0.011^j	0.5±0.012^a	19.91±0.64^a	0.1±0.035^e
	B₁	4.67±0.22^e	0.29±0.018^ab	438±11.14^a	0.57±0.015^f	0.5±0.012^a	8.13±0.18^hi	-0.02±0.012^g
	B₂	5.87±0.19^a	0.28±0.018^ab	417.33±11.06^b	0.3±0.013ⁱ	0.51±0.011^a	19.33±0.42^b	0.03±0.01^fg
	B₃	5.18±0.29^d	0.22±0.016^f	368±10.54^d	0.71±0.013^c	0.5±0.014^a	7.31±0.28^j	0.14±0.03^1e
	C₁	5.33±0.15^cd	0.26±0.012^bcd	294.33±8.5^fgh	0.3±0.012ⁱ	0.48±0.011^b	17.53±0.64^c	0.32±0.035^bcd
	C₂	5.95±0.1^a	0.29±0.017^a	277.33±10.5^hi	0.71±0.013^c	0.5±0.014^ab	8.34±0.16^h	0.36±0.035^b
	C₃	4.67±0.18^e	0.28±0.014^abc	274.33±9.5ⁱ	0.32±0.015ⁱ	0.49±0.013^ab	14.46±0.09^f	0.36±0.06^b
	D₁	5.55±0.18^bc	0.27±0.012^abc	288±9^ghi	0.94±0.009^a	0.48±0.012^b	5.91±0.14^k	0.33±0.05^bc
	D₂	5.75±0.19^ab	0.26±0.013^bcd	289.33±11.02^ghi	0.36±0.011^h	0.43±0.013^cd	15.8±0.47^d	0.33±0.045^bc
	D₃	5.11±0.18^d	0.26±0.018^cde	291.33±10.26^fghi	0.61±0.01^e	0.41±0.011^d	8.33±0.25^h	0.32±0.035^bcd
	E₁	3.96±0.13^f	0.27±0.014^abc	301±10.54^efg	0.92±0.012^a	0.43±0.011^c	4.28±0.09^l	0.3±0.045^bcd
	E₂	5.23±0.13^cd	0.26±0.018^bcd	308±9.54^ef	0.54±0.01^g	0.41±0.011^d	9.72±0.07^g	0.28±0.035^cd
	E₃	5.83±0.21^ab	0.24±0.022^def	316±11.53^e	0.76±0.01^b	0.43±0.011^cd	7.63±0.23^ij	0.26±0.046^d
	CK	2.96±0.19^g	0.18±0.016^g	224.33±12.06^j	0.36±0.013^h	0.31±0.012^e	8.13±0.23^hi	0.48±0.046^a

组别	指标	特征向量
组别	指标	1	2	3
TY	Pn	0.219	0.21	-0.407
	Gs	0.228	0.133	-0.038
	Ci	0.209	-0.257	0.265
	VpdL	0.065	0.34	0.962
	Tr	0.225	-0.176	-0.185
	WUE	0.113	0.425	-0.313
	LS	-0.209	0.254	-0.27
	特征值	3.918	1.925	0.732
	贡献率/%	55.973	27.502	10.456
	累计贡献率/%	55.973	83.475	93.931
CY	Pn	0.183	0.154	0.499
	Gs	0.184	0.223	0.403
	Ci	0.257	0.007	-0.464
	VpdL	0.267	0.108	0.096
	Tr	-0.068	0.524	-0.09
	WUE	0.151	-0.437	0.28
	LS	-0.256	-0.009	0.469
	特征值	3.361	1.814	1.062
	贡献率/%	48.016	25.918	15.174
	累计贡献率/%	48.016	73.934	89.108