Research on Control Effect of Streptomyces costaricanus Strain A-m1 on Wheat Scab

doi:10.11924/j.issn.1000-6850.casb2024-0192

Abstract

Abstract:

The control effects of Streptomyces costaricanus strain A-m1 on wheat scab were studied in order to lay a foundation for the development of biocontrol agents for wheat production. The effects of A-m1 on spore germination and mycelial growth of wheat scab pathogen were determined, the genetic and enzymatic basis of the bacteriostatic effect of strain A-m1 was analyzed, and the control effect of spraying A-m1 bacterial liquid at flowering stage and applying A-m1 solid bacterial fertilizer at sowing stage on wheat scab was explored. The findings revealed that strain A-m1 exerted a potent inhibitory influence on both the conidial germination and mycelial expansion of Fusarium graminearum. The genomic of A-m1 encoded genes for the synthesis of streptomycin and tetracycline antibiotics, as well as genes such as casein, β-1, 3-glucanase, cellulase, and chitinase. They had the effect of inhibiting or decomposing pathogenic bacteria, and further biochemical analysis verified the secretion of the four enzymes. Spraying A-m1 fermentation broth at wheat flowering stage had a control effect of 52.28 % on wheat scab, a level of efficacy comparable to that of a 1000-fold dilution of 80% carbendazim solution. The application of A-m1 bacterial fertilizer substituting a portion of the traditional compound fertilizer at the sowing stage, led to a marked increase in the activity of defense enzymes, including polyphenol oxidase, peroxidase, catalase, and phenylalanine ammonia-lyase. At the same time, the content of malondialdehyde was lower, and the control effect on wheat scab was 57.18 %. In this study, the control effect of strain A-m1 on wheat scab was clarified, and the underlying mechanisms of its protective action had been partially dissected. Field experiments also confirmed the control effect of A-m1 fermentation broth spraying and strain A-m1 bacterial fertilizer application on wheat scab during sowing period, which laid a good foundation for the reduction of chemical fertilizers and pesticides in wheat production.

Key words: Streptomyces costaricanus, induced resistance, antibacterial enzyme, wheat scab, microbial fertilizer

LI Yongli, CHEN Lei, ZHOU Zhou, GENG Shubao, QIAO Li, ZHANG Fangmei, CHEN Lijun, WANG Lijuan. Research on Control Effect of Streptomyces costaricanus Strain A-m1 on Wheat Scab[J]. Chinese Agricultural Science Bulletin, 2024, 40(30): 128-134.

Figures/Tables 6

References 28

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处理组	病情指数	防治效果/%
CK	52.81±3.69 b	－
A-m1发酵液	28.20±2.26 a	52.28
80%多菌灵1000倍液	30.59±1.96a	52.23

处理组	病情指数	防治效果/%
CK	52.81±3.69 b	－
A-m1发酵液	28.20±2.26 a	52.28
80%多菌灵1000倍液	30.59±1.96a	52.23