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Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (11): 156-156.

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Development of Colloidal Gold Immunostrip for Rapid Detection of Chloramphenicol Residue in Food of Animal Origin

Yang Ting, Wang Shuting, Guo Yirong, Wang Meiying, Zhu Guonian   

  • Online:2007-11-05 Published:2007-11-05

Abstract: Monoclonal antibody (McAb) against Chloramphenicol (CAP) was prepared by means of hybridoma technique, and an immunostrip for the determination of CAP was developed based on competitive colloidal-gold immunochromatographic assay. Results of the study indicated that the McAb had good affinity and high specificity to CAP. The gold-labeled immunostrip, was suitable for rapid detection of CAP residues in food of animal origin, with the detection limit 1.5μg/kg in shrimp or honey and 3μg/kg in milk, respectively, and the test time was only 5-8 min.

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