鸭POU1F1基因第2内含子多态性及其遗传效应

doi:10.11924/j.issn.1000-6850.2013-1986

中国农学通报 ›› 2014, Vol. 30 ›› Issue (8): 7-11.doi: 10.11924/j.issn.1000-6850.2013-1986

所属专题：生物技术；畜牧兽医

鸭POU1F1基因第2内含子多态性及其遗传效应

林秋娟何桂娟黄守婷钟志新周世业黄种彬肖天放

收稿日期:2013-07-20 修回日期:2013-10-15 出版日期:2014-03-15 发布日期:2014-03-15
基金资助:
福建省重大科技专项资助项目 “福建省水禽遗传多样性的研究 ” (2006NZ003-3 )。

Polymorphism of POU1F1 Gene Intron2 and Its Genetic Effects in Duck

XIAO Tian-Fang

Received:2013-07-20 Revised:2013-10-15 Online:2014-03-15 Published:2014-03-15

摘要/Abstract

摘要： 为探究褐色菜鸭POU1F1基因的多态性及其遗传效应，本试验采用PCR-SSCP方法检测了褐色菜鸭POU1F1基因的多态性，分析了多态位点不同基因型与褐色菜鸭生产性状的关联性。结果表明：POU1F1基因第2内含子存在3种基因型即AA型、Aa型和aa型，序列比对存在2个突变位点即A3980T和A3988G，该位点属低度多态，?2检验结果表明该基因座位处于Hardy-Weinberg平衡(P>0.05)；关联分析显示：AA基因型个体的蛋形指数和四周龄体重均显著高于aa型(P＜0.05)，但三种基因型对其他性状无显著影响(P>0.05)。推测该片段的多态位点可能对四周龄体重和蛋形指数有一定的影响，POU1F1基因第2内含子可作为褐色菜鸭生产性能的优势分子标记。

关键词: 效果, 效果

Abstract: This study aimed to detect the polymorphism of POU1F1 gene and its genetic effects on Brown Tsaiya Duck. PCR-SSCP method was applied in this study to detect the polymorphisms of POU1F1 gene in Brown Tsaiya Duck, and analyze the association of polymorphisms with production traits of Brown Tsaiya Duck. The results showed that POU1F1 intron 2 was consisted of 3 genotypes (AA, Aa, aa). 2 SNPs loci (A3980T, A3988G) were detected. This site belongs to low polymorphism, and the result of ?2 indicated that this site was in state of Hardy-Weinberg equilibrium. Both egg shape index and body weight at 4th week of AA genotype were significantly higher than that of aa (P<0.05), while three kinds of genotypes had no significant effect on other indexes (P>0.05). The results suggested that the SNPs had certain effect on egg shape index and body weight at 4th week, and might be a good marker for production traits in Brown Tsaiya Duck.

林秋娟何桂娟黄守婷钟志新周世业黄种彬肖天放. 鸭POU1F1基因第2内含子多态性及其遗传效应[J]. 中国农学通报, 2014, 30(8): 7-11.

XIAO Tian-Fang. Polymorphism of POU1F1 Gene Intron2 and Its Genetic Effects in Duck[J]. Chinese Agricultural Science Bulletin, 2014, 30(8): 7-11.

参考文献

[1] Steinfelder H L, Radovick S. Hormonal regulation of the Thyrotrop in subunit gene by phosphory lation of the pituitary specific transcription factor (PIT- I) [J].Proc Nat A cad Sci USA,1992,89: 5942-5945.
[2] Cohenle, Wondisfordfe, Radovick S. Role of Pit- 1 in the gene expression of growth hormone，prolactin, and thyrotropin[J]. Endocrinology & Metabolism Clinics of North America,1996,25(3): 523-540.
[3] Hendriks S T, Egeman B I, Augustijin K D, et al. Combinedpituitary hormone deficiency caused by compound heterozy gosity for twonovel mutations in the POU domain of the Pit/POU1F1 gene [J]. The Jour nal of Clinical Endocrinology & Metabolism,2001,86(4):1545-1550.
[4] Moody D E, Porpp D, Baren dse W. Restriction fragment length polymorphism in amplification products of the bovine Pit- 1 gene and assignment of Pit- 1 bovine chromosome1[J].Animal Genetic, 1995,26(1):45- 47.
[5] Woollard J, S chmit Z C B, Freeman A E, et al. Hinf I polymorphism at the bovine Pit1 ocus [J].J Anim Sci,1994,72(12):3267.
[6] Tanaka M, Yamamoto I, Ohkubo T, et al. cDNA cloning and developmental alterations inGene expression of the two Pit-1/GHF- 1transcription factors in The chicken pituitary[J].General and Comparative Endocrinology,1999,114:441-4481.
[7] Li H B, Cao H H, Zheng Y M. The current study status of Pit- 1 gene in humans， mice and pigs[J].Hereditas (Bei- jing),2003,23(6): 605-6081.
[8] 朱志明, 陈辉,李盛霖.半番鸭 pou1f1基因序列的克隆与生物信息学分析[J].福建畜牧兽医,2010,32(1):1-4.
[9] Stancekova K, Vasicek D, Peskovicova D. Effcet of genetic varibility of the porcine pituitaryspecific transcription factor (PIT-1) on carcass traints in pig[J].Animal Genetics,1999,30(4):313-315.
[10] Yu T P, Rothschild M F, Tuggle C K. A MspI restriction fragment length polymorphism at the swine PIT- 1 locus[J].Journal Animal Science,1993,71(8):2275.
[11] Anderson B, Rosonfeld M G. Pit- 1 determines cell types during development of the anterior pituitaru gland[J].Amodel for Transcription Biol Chem,1994,269(47):29335-29338.
[12] Leevwen I S L, Mol J A, Kooistra H S, et al. Cloning of the canine gene encoding transcription factor PIT- 1 and its exclusion as candidate gene in a canine model of pituitarydwarfism[J]. Mammalian Genome,2000,11(1):31-36.
[13] Lee N C, Tsai W Y, Peng S F, et al. Congenital Hypopituitarism due to POU1F1 Gene Mutation[J].J Formos Med Assoc,2011,110(1):58- 61.
[14] 李俊营,詹凯,许月英,等.巢湖鸭生长素级基因和垂体特异性转录因子 1 基因的 PCR-SSCP 检测[J].中国畜牧兽医,2009,36(5):107- 111.
[15] 姜润深,杨宁.家禽垂体特异转录因子 POU1F 研究进展[J].遗传, 2004(6):957-961.
[16] Nie Q H, Fang M X, Xie L. The PIT-1 gene polymorphisms were associcated with chicken growth[J].BMC Genetic,2008,9(20):1-5.
[17] Xu H Y, Wang Y, Liu Y P. Polymorphisms and expression of the chicken POU1F1 gene associated with carcass traits[J].MBR,2012 (39):8263-8271.
[18] Yan L J, Fang X T, Liu Y, et al. Effects of single and combined genotypes of MC4R and POU1F1 genes on two production traits in Langshan chicken[J].Mol Biol Rep,2013(40):4645-4650.
[19] 李国辉,魏岳,张学余.鸡 GH和 POU1F1基因多态性及其基因聚合对产蛋数的影响[J].湖南农业大学学报,2010,36(4):445-448.
[20] 胡玉萍,李国辉,王金玉.京海黄鸡POU1F1基因多态性及其与生长性能的遗传效应分析[J].中国家禽,2008,30(11):20-22.

鸭POU1F1基因第2内含子多态性及其遗传效应

Polymorphism of POU1F1 Gene Intron2 and Its Genetic Effects in Duck

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