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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 268-272.

所属专题： 生物技术； 智慧农业

• 植物保护 农药 • 上一篇    下一篇

应用SAS软件优化武夷菌素产生菌发酵培养基

程艳 张克诚 赵明富 孙蕾 崔增杰 檀贝贝   

  • 收稿日期:2010-04-28 修回日期:2010-07-21 出版日期:2010-11-05 发布日期:2010-11-05
  • 基金资助:

    公益性行业（农业）科研专项资金项目：“生态康复型农田绿色控害技术研究”;国家科技支撑计划课题：“农林重大生物灾害防控技术研究”。

Optimization of Fermentation Culture Medium for Producing Strain of Wuyiencin by SAS software

  • Received:2010-04-28 Revised:2010-07-21 Online:2010-11-05 Published:2010-11-05

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摘要：

武夷菌素是由不吸水链霉菌武夷变种(Streptomyces ahygroscopicus var.wuyiensis)产生的一种农用抗生素，对多种作物的真菌病害防治效果显著。为进一步提高武夷菌素产生菌的产量、降低其生产成本，应用SAS软件提供的Plackett-Burman设计法和响应面分析法对该菌株的发酵培养基进行优化组合，以期找到适宜武夷菌素产生菌C-75生产的最佳发酵培养基配方。应用Plackett-Burman设计法从9种培养基成分中筛选出玉米粉和碳酸钙为两个重要因素；通过响应面回归分析结果建立响应面回归模型：Y=5826.99-91.56X2-180.69X8-194.31X22 -151.25X2X8-205.31X82。回归模型预测当玉米粉和碳酸钙的含量分别为2.314 g和0.205 g（50 ml培养基装量）时，该模型能取到最大值。摇瓶发酵实验结果表明，该模型稳定点处的实际效价平均值为5837 μg/ml，菌株C-75经优化培养基发酵后的效价较之前提高了5.59%。

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Abstract:

Abstract: Wuyiencin, an agricultural antibiotic, was produced from the Streptomyces ahygroscopicus var. wuyiensis. Wuyiencin used as fungicide has strong inhibitant effects and obvious control effects on many fungi pathogenic of crops. The aim of this research is to increase production of producing strains and decrease production costs of Wuyiencin. By using Plackett-Burman Design and Response Surface Method which provided by SAS software to optimize the fermentation culture medium of strain C-75.Corn flour and CaCO3 were chosen as the major components from nine compositions of medium. Then the regression model of RSA was established by Response Surface Method: Y=5826.99-91.56X2-180.69X8- 194.31X22-151.25X2X8-205.31X82.It was predicted that when the concentrations of corn flour and CaCO3 were 2.314g and 0.205g respectively(the volume of medium is 50ml), the regression model exist maximum. The average tieter of Wuyiencin was determined 5837μg/ml actually by shake-flask. The Wuyiencin production was increased by 38.53% when strain C-75 was cultivated in the optimum medium instead of the original one.

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