Effect of β-mercaptoethanol on Total RNA Quantity of Mango Pruning Wound Buds in Different Periods

doi:10.11924/j.issn.1000-6850.casb16070085

Abstract

Abstract: It is difficult to extract total RNA from the pruning wound buds of mango since those buds are rich in phenols which are easily oxidized and hardly separated from the nucleic acid. In order to extract high quality RNA which was suitable for real-time fluorescent quantitative PCR. The influence of β-mercaptoethanol on the quality of RNA extraction was discussed by adding an appropriate amount of β-mercaptoethanol into the cell lysis buffers of quick RNA isolation kit which was made by HUAYUEYANG BIOTECHNOLOGY (BEIJING) CO. LTD. The results showed that adding about 300 μl β-mercaptoethanol into the cell lysis buffers, which could eliminate the interference impurities such as phenols, pigments, et al. Total RNA isolated by this method was of high quality with a full strip, and the result of RT-PCR indicated that it was able to be used for later molecular manipulations.

CLC Number:

S859.84

周玲,赵凯 and 彭磊. Effect of β-mercaptoethanol on Total RNA Quantity of Mango Pruning Wound Buds in Different Periods[J]. Chinese Agricultural Science Bulletin, 2017, 33(10): 83-86.

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