Abstract: The accumulated temperature is a kind of important eco- environmental resource and climatic resource. Knowing the temporal and spatial variation rules of accumulated temperature is significant to guide agricultural production and eco-environmental construction. Based on the accumulated temperature ground data of 34 automatic weather stations from 1960 to 2016 in Chongqing, the authors analyzed the temporal and spatial variation rules of ≥10℃ accumulated temperature, by using Mann-Kendal mutation test and Morlet wavelet cycle analysis and other meteorological statistical methods. The results showed that: the accumulated temperature distributed unevenly in Chongqing, there were 3 regions with large distribution differences along the Yangtze River and Wujiang River; in most areas of Chongqing, the annual average accumulated temperature was between 5500℃ and 6500℃; during 1960-1980, the temperature change in Chongqing was mainly manifested by a warming trend; during 1981- 2000, the accumulated temperature was significantly reduced; after 2000, there were positive and negative phases; the accumulated temperature changed significantly fast, and the climate became more abnormal; before 1980s, the amplitude of the accumulated temperature was large in area 1 of Chongqing, after 1980s, the amplitude decreased significantly; the variation trend of accumulated temperature and change rules and the amplitude were basically consistent in area 2 and 3. Jiangjin, Fengdu, Wanzhou and the other regions of Chongqing have abundant accumulated temperature heat resources which can meet the requirement of three cropping a year system, and can carry out the pilot experiment of this cultivation system.