Oviductus Ranae and Oviducts of Bufo Gargarizans: Proteomics Analysis

doi:10.11924/j.issn.1000-6850.casb20190600321

Abstract

Abstract:

The aim is to select differentially expressed proteins in oviducts of Chinese Brown frog (OR) and Bufo gargarizans (OBG), and then to find the relationship between these differentially expressed proteins and growth environment, hibernation as well as developments. The proteomics database of OR and OBG were established using iTRAQ technology combined with MS. By comparing the two proteomics database, GO annotation and bioinformatics analysis was conducted for the selected proteins. The results showed that there were 68 differential proteins, including 61 up-regulated proteins and 7 down-regulated proteins. In OR, 7 high expressed proteins were divided into 5 categories, mucins (PREDICTED: mucin-5AC-like, PREDICTED: similar to mucin, mCG142254, partial), collagen alpha-1(III) chain, PREDICTED: a-kinase anchor protein 13-like, PREDICTED: IgGFc-binding protein-like, and annexin A1, which mainly participated in immunomodulation and development. At same time, antifreeze ingredients were mainly mucins in OR, however which were lectin in OBG. 7 high expressed proteins were important proteins in OR, which could be used to distinguish OBG from OR. Exceptional swelling capacity of OR was closely related to a large amount of mucins.

Key words: oviductus ranae (OR), oviducts of Bufo gargarizans (OBG), differentially expressed proteins, isobaric tags for relative and absolute quantitation (iTRAQ)

CLC Number:

S859

Su Wenjie, Zhang He, Li Jiangdan, Dong Ying, Li Xiangyan, Song Bailin, Zhao Daqing. Oviductus Ranae and Oviducts of Bufo Gargarizans: Proteomics Analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 126-135.

Figures/Tables 5

NO.			Accession		Nr	Description	$ΑΒ ˉ$ /REF		D/REF		D/ $ΑΒ ˉ$ ratio		D/ $ΑΒ ˉ$ ratio Significance A
1			Unigene70397_OR		gi\|148226545\|	hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta	0.217		1.651		7.608		0.000
2			Unigene63396_OR		gi\|118573094\|	Ovochymase-2	0.288		1.739		6.038		0.000
3			Unigene56086_B		gi\|126328955\|	PREDICTED: similar to NGEP long	0.359		2.125		5.919		0.000
4			Unigene77724_OR		gi\|49117916\|	LOC443585 protein, partial	0.391		1.951		4.990		0.000
5			Unigene20044_OR		gi\|224073015\|	PREDICTED: similar to FK506 binding protein 15, 133kDa	0.461		1.951		4.232		0.000
6			Unigene98709_B		gi\|148235835\|	protein FAM3C precursor	0.264		1.488		5.636		0.000
7			Unigene17821_B		gi\|47575826\|	fructose-bisphosphate aldolase C	0.336		1.691		5.040		0.000
8			Unigene48987_OR		gi\|301604422\|	PREDICTED: protein FAM123B-like	0.414		2.013		4.868		0.000
9			Unigene18502_OR		gi\|148233882\|	keratin 15, gene 1 S homeolog	0.479		2.073		4.332		0.000
10			Unigene36139_OR		gi\|158021651\|	actin	0.354		1.462		4.130		0.000
11			Unigene62140_OR		gi\|226372874\|	tropomyosin-1 alpha chain	0.451		1.735		3.847		0.000
12			Unigene10323_OR		gi\|113931584\|	acetoacetyl-CoA synthetase	0.619		1.486		2.403		0.000
13			Unigene59207_OR		gi\|85682734\|	glutaminyl cyclase precursor	0.525		2.135		4.067		0.000
14			Unigene83348_B		gi\|301603710\|	PREDICTED: tenascin-like	0.618		2.002		3.242		0.000
15			Unigene32357_OR		gi\|45361679\|	eukaryotic translation initiation factor 3 subunit C	0.592		1.777		3.002		0.000
16			Unigene9059_OR		gi\|163914875\|	extracellular superoxide dismutase [Cu-Zn] precursor	0.524		2.023		3.861		0.000
17			Unigene24604_OR		gi\|225707616\|	ADP-ribosylation factor-like protein 1	0.435		1.384		3.182		0.000
18			Unigene22925_B		gi\|37805193\|	MGC68756 protein	0.384		1.237		3.226		0.000
19			Unigene83445_B		gi\|147899623\|	thioredoxin domain containing 12 (endoplasmic reticulum) S homeolog precursor	0.523		1.851		3.543		0.000
20			Unigene49111_OR		gi\|291415601\|	PREDICTED: cofilin 1 (non-muscle)-like	0.459		1.400		3.053		0.000
21			Unigene89250_B		gi\|48735027\|	Atp1a1a.1 protein	0.538		1.457		2.708		0.000
22			Unigene30790_B		gi\|47575816\|	ectonucleoside triphosphate diphosphohydrolase 2	0.515		1.776		3.452		0.000
23			Unigene76910_B		gi\|297492017\|	PREDICTED: calmodulin 2-like	0.457		1.396		3.055		0.001
24			Unigene92646_B		gi\|147903244\|	RAB15, member RAS oncogene family S homeolog	0.508		1.281		2.522		0.001
25			Unigene76458_OR		gi\|171847235\|	DEAD (Asp-Glu-Ala-Asp) box polypeptide 18	0.235		0.782		3.328		0.001
26			Unigene101349_B		gi\|46048885\|	collagen alpha-1(V) chain precursor	0.455		1.375		3.022		0.001
27			Unigene41554_OR		gi\|301786150\|	PREDICTED: coatomer subunit alpha-like isoform 2	0.357		1.178		3.304		0.002
28			Unigene61562_OR		gi\|301627325\|	PREDICTED: calpain small subunit 1	0.476		1.354		2.845		0.002
29			Unigene69856_B		gi\|52345948\|	V-type proton ATPase subunit G 1	0.656		2.019		3.080		0.002
30			Unigene36206_B		gi\|224045171\|	PREDICTED: thioredoxin domain containing 5	0.551		1.524		2.768		0.003
31			Unigene98750_B		gi\|301605782\|	PREDICTED: voltage-dependent T-type calcium channel subunit alpha-1H-like	0.520		1.505		2.894		0.003
32		Unigene67246_B		gi\|291395962\|		PREDICTED: tubulin beta chain		0.533		1.381		2.591		0.003
33		Unigene72828_OR		gi\|148225833\|		glycerol-3-phosphate dehydrogenase 1 L homeolog		0.648		1.606		2.478		0.007
34		Unigene85920_B		gi\|126290220\|		PREDICTED: heat shock protein HSP 90-alpha isoform X2		0.655		1.569		2.397		0.007
35		Unigene1604_OR		gi\|301611116\|		PREDICTED: filamin-B-like		0.559		1.528		2.733		0.008
	36	Unigene68308_OR		gi\|226372846\|		Receptor expression-enhancing protein 5		0.605		1.515		2.504		0.009
	37	Unigene29753_OR		gi\|89515102\|		heat shock protein 90		0.615		1.607		2.615		0.010
	38	Unigene96724_B		gi\|126326088\|		PREDICTED: similar to 90-kDa heat shock protein		0.595		1.319		2.217		0.011
	39	Unigene94902_B		gi\|297669245\|		PREDICTED: LOW QUALITY PROTEIN: ras-associated and pleckstrin homology domains-containing protein 1-like		0.595		1.622		2.728		0.012
	40	Unigene29074_OR		gi\|113931560\|		endoplasmin precursor		0.625		1.359		2.174		0.012
	41	Unigene73852_B		gi\|301298989\|		actin		0.578		1.391		2.407		0.014
	42	Unigene48455_B		gi\|301792224\|		PREDICTED: elongation factor 1-alpha 1-like		0.448		1.222		2.731		0.014
	43	Unigene65461_OR		gi\|226371966\|		GTP-binding nuclear protein Ran		0.547		1.390		2.541		0.018
	44	Unigene96782_B		gi\|148234370\|		peroxiredoxin 5 L homeolog precursor		0.617		1.305		2.115		0.020
	45	Unigene43582_OR		gi\|148726784\|		annexin A4		0.694		1.672		2.409		0.020
	46	Unigene40654_OR		gi\|292613778\|		PREDICTED: si:ch211-102c2.8		0.651		1.799		2.763		0.022
	47	Unigene47846_B		gi\|147899037\|		malate dehydrogenase 2 L homeolog		0.557		1.410		2.534		0.022
	48	Unigene54017_OR		gi\|147899962\|		lectin		0.675		1.720		2.548		0.026
	49	Unigene78258_B		gi\|66910271\|		Actr3 protein		0.644		1.513		2.349		0.030
	50	Unigene77639_B		gi\|281427350\|		ATP synthase subunit g, mitochondrial		0.712		1.844		2.590		0.031
	51	Unigene42710_OR		gi\|6980088\|		inner-ear cytokeratin		0.484		1.126		2.329		0.032
	52	Unigene30738_OR		gi\|301771656\|		PREDICTED: alcohol dehydrogenase class-3		0.631		1.455		2.306		0.033
	53	Unigene29744_B		gi\|69219613\|		tropomyosin beta, partial		0.624		1.417		2.271		0.034
	54	Unigene86687_B		gi\|66267684\|		DJ-1		0.762		1.477		1.938		0.037
	55	Unigene92147_B		gi\|112418924\|		Dnl2 protein, partial		0.671		1.573		2.346		0.037
	56	Unigene45972_OR		gi\|76779672\|		MGC132180 protein		0.691		1.439		2.084		0.037
	57	Unigene93505_B		gi\|58332368\|		cytosolic 10-formyltetrahydrofolate dehydrogenase		0.790		1.835		2.324		0.038
	58	Unigene69_B		gi\|147906126\|		peptidyl arginine deiminase, type II S homeolog		0.593		1.369		2.311		0.041
	59	Unigene65493_OR		gi\|148232533\|		chaperonin containing TCP1 subunit 2 S homeolog		0.664		1.544		2.327		0.046
	60	Unigene97210_B		gi\|62857923\|		ras-related protein Rap-2c		0.754		1.476		1.959		0.046
	61	Unigene25198_OR		gi\|34221745\|		triosephosphate isomerase, partial		0.619		1.233		1.994		0.047

NO.			Accession		Nr	Description	$ΑΒ ˉ$ /REF		D/REF		D/ $ΑΒ ˉ$ ratio		D/ $ΑΒ ˉ$ ratio Significance A
1			Unigene70397_OR		gi\|148226545\|	hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta	0.217		1.651		7.608		0.000
2			Unigene63396_OR		gi\|118573094\|	Ovochymase-2	0.288		1.739		6.038		0.000
3			Unigene56086_B		gi\|126328955\|	PREDICTED: similar to NGEP long	0.359		2.125		5.919		0.000
4			Unigene77724_OR		gi\|49117916\|	LOC443585 protein, partial	0.391		1.951		4.990		0.000
5			Unigene20044_OR		gi\|224073015\|	PREDICTED: similar to FK506 binding protein 15, 133kDa	0.461		1.951		4.232		0.000
6			Unigene98709_B		gi\|148235835\|	protein FAM3C precursor	0.264		1.488		5.636		0.000
7			Unigene17821_B		gi\|47575826\|	fructose-bisphosphate aldolase C	0.336		1.691		5.040		0.000
8			Unigene48987_OR		gi\|301604422\|	PREDICTED: protein FAM123B-like	0.414		2.013		4.868		0.000
9			Unigene18502_OR		gi\|148233882\|	keratin 15, gene 1 S homeolog	0.479		2.073		4.332		0.000
10			Unigene36139_OR		gi\|158021651\|	actin	0.354		1.462		4.130		0.000
11			Unigene62140_OR		gi\|226372874\|	tropomyosin-1 alpha chain	0.451		1.735		3.847		0.000
12			Unigene10323_OR		gi\|113931584\|	acetoacetyl-CoA synthetase	0.619		1.486		2.403		0.000
13			Unigene59207_OR		gi\|85682734\|	glutaminyl cyclase precursor	0.525		2.135		4.067		0.000
14			Unigene83348_B		gi\|301603710\|	PREDICTED: tenascin-like	0.618		2.002		3.242		0.000
15			Unigene32357_OR		gi\|45361679\|	eukaryotic translation initiation factor 3 subunit C	0.592		1.777		3.002		0.000
16			Unigene9059_OR		gi\|163914875\|	extracellular superoxide dismutase [Cu-Zn] precursor	0.524		2.023		3.861		0.000
17			Unigene24604_OR		gi\|225707616\|	ADP-ribosylation factor-like protein 1	0.435		1.384		3.182		0.000
18			Unigene22925_B		gi\|37805193\|	MGC68756 protein	0.384		1.237		3.226		0.000
19			Unigene83445_B		gi\|147899623\|	thioredoxin domain containing 12 (endoplasmic reticulum) S homeolog precursor	0.523		1.851		3.543		0.000
20			Unigene49111_OR		gi\|291415601\|	PREDICTED: cofilin 1 (non-muscle)-like	0.459		1.400		3.053		0.000
21			Unigene89250_B		gi\|48735027\|	Atp1a1a.1 protein	0.538		1.457		2.708		0.000
22			Unigene30790_B		gi\|47575816\|	ectonucleoside triphosphate diphosphohydrolase 2	0.515		1.776		3.452		0.000
23			Unigene76910_B		gi\|297492017\|	PREDICTED: calmodulin 2-like	0.457		1.396		3.055		0.001
24			Unigene92646_B		gi\|147903244\|	RAB15, member RAS oncogene family S homeolog	0.508		1.281		2.522		0.001
25			Unigene76458_OR		gi\|171847235\|	DEAD (Asp-Glu-Ala-Asp) box polypeptide 18	0.235		0.782		3.328		0.001
26			Unigene101349_B		gi\|46048885\|	collagen alpha-1(V) chain precursor	0.455		1.375		3.022		0.001
27			Unigene41554_OR		gi\|301786150\|	PREDICTED: coatomer subunit alpha-like isoform 2	0.357		1.178		3.304		0.002
28			Unigene61562_OR		gi\|301627325\|	PREDICTED: calpain small subunit 1	0.476		1.354		2.845		0.002
29			Unigene69856_B		gi\|52345948\|	V-type proton ATPase subunit G 1	0.656		2.019		3.080		0.002
30			Unigene36206_B		gi\|224045171\|	PREDICTED: thioredoxin domain containing 5	0.551		1.524		2.768		0.003
31			Unigene98750_B		gi\|301605782\|	PREDICTED: voltage-dependent T-type calcium channel subunit alpha-1H-like	0.520		1.505		2.894		0.003
32		Unigene67246_B		gi\|291395962\|		PREDICTED: tubulin beta chain		0.533		1.381		2.591		0.003
33		Unigene72828_OR		gi\|148225833\|		glycerol-3-phosphate dehydrogenase 1 L homeolog		0.648		1.606		2.478		0.007
34		Unigene85920_B		gi\|126290220\|		PREDICTED: heat shock protein HSP 90-alpha isoform X2		0.655		1.569		2.397		0.007
35		Unigene1604_OR		gi\|301611116\|		PREDICTED: filamin-B-like		0.559		1.528		2.733		0.008
	36	Unigene68308_OR		gi\|226372846\|		Receptor expression-enhancing protein 5		0.605		1.515		2.504		0.009
	37	Unigene29753_OR		gi\|89515102\|		heat shock protein 90		0.615		1.607		2.615		0.010
	38	Unigene96724_B		gi\|126326088\|		PREDICTED: similar to 90-kDa heat shock protein		0.595		1.319		2.217		0.011
	39	Unigene94902_B		gi\|297669245\|		PREDICTED: LOW QUALITY PROTEIN: ras-associated and pleckstrin homology domains-containing protein 1-like		0.595		1.622		2.728		0.012
	40	Unigene29074_OR		gi\|113931560\|		endoplasmin precursor		0.625		1.359		2.174		0.012
	41	Unigene73852_B		gi\|301298989\|		actin		0.578		1.391		2.407		0.014
	42	Unigene48455_B		gi\|301792224\|		PREDICTED: elongation factor 1-alpha 1-like		0.448		1.222		2.731		0.014
	43	Unigene65461_OR		gi\|226371966\|		GTP-binding nuclear protein Ran		0.547		1.390		2.541		0.018
	44	Unigene96782_B		gi\|148234370\|		peroxiredoxin 5 L homeolog precursor		0.617		1.305		2.115		0.020
	45	Unigene43582_OR		gi\|148726784\|		annexin A4		0.694		1.672		2.409		0.020
	46	Unigene40654_OR		gi\|292613778\|		PREDICTED: si:ch211-102c2.8		0.651		1.799		2.763		0.022
	47	Unigene47846_B		gi\|147899037\|		malate dehydrogenase 2 L homeolog		0.557		1.410		2.534		0.022
	48	Unigene54017_OR		gi\|147899962\|		lectin		0.675		1.720		2.548		0.026
	49	Unigene78258_B		gi\|66910271\|		Actr3 protein		0.644		1.513		2.349		0.030
	50	Unigene77639_B		gi\|281427350\|		ATP synthase subunit g, mitochondrial		0.712		1.844		2.590		0.031
	51	Unigene42710_OR		gi\|6980088\|		inner-ear cytokeratin		0.484		1.126		2.329		0.032
	52	Unigene30738_OR		gi\|301771656\|		PREDICTED: alcohol dehydrogenase class-3		0.631		1.455		2.306		0.033
	53	Unigene29744_B		gi\|69219613\|		tropomyosin beta, partial		0.624		1.417		2.271		0.034
	54	Unigene86687_B		gi\|66267684\|		DJ-1		0.762		1.477		1.938		0.037
	55	Unigene92147_B		gi\|112418924\|		Dnl2 protein, partial		0.671		1.573		2.346		0.037
	56	Unigene45972_OR		gi\|76779672\|		MGC132180 protein		0.691		1.439		2.084		0.037
	57	Unigene93505_B		gi\|58332368\|		cytosolic 10-formyltetrahydrofolate dehydrogenase		0.790		1.835		2.324		0.038
	58	Unigene69_B		gi\|147906126\|		peptidyl arginine deiminase, type II S homeolog		0.593		1.369		2.311		0.041
	59	Unigene65493_OR		gi\|148232533\|		chaperonin containing TCP1 subunit 2 S homeolog		0.664		1.544		2.327		0.046
	60	Unigene97210_B		gi\|62857923\|		ras-related protein Rap-2c		0.754		1.476		1.959		0.046
	61	Unigene25198_OR		gi\|34221745\|		triosephosphate isomerase, partial		0.619		1.233		1.994		0.047

NO.	Accession	Nr-ID	Description	$ΑΒ ˉ$ /REF	$CD ˉ$ /REF	$CD ˉ$ / $ΑΒ ˉ$ ratio	$CD ˉ$ / $ΑΒ ˉ$ ratio Significance A
1	Unigene71408_B	gi\|296490750\|	collagen alpha-1(III) chain	1.166	0.048	0.041	0.032
2	Unigene36652_B	gi\|148686175\|	mCG142254, partial	3.824	0.190	0.050	0.038
3	Unigene23627_B	gi\|301773364\|	PREDICTED: a-kinase anchor protein 13-like	4.674	0.233	0.050	0.041
4	Unigene50328_B	gi\|301613162\|	PREDICTED: IgGFc-binding protein-like	4.192	0.322	0.077	0.043
5	Unigene31388_B	gi\|301613160\|	PREDICTED: mucin-5AC-like	4.003	0.364	0.091	0.046
6	Unigene35953_B	gi\|283837861\|	annexin A1	1.515	0.139	0.092	0.048
7	Unigene35972_B	gi\|118091320\|	PREDICTED: similar to mucin	3.318	0.364	0.110	0.048

NO.	Accession	Nr-ID	Description	$ΑΒ ˉ$ /REF	$CD ˉ$ /REF	$CD ˉ$ / $ΑΒ ˉ$ ratio	$CD ˉ$ / $ΑΒ ˉ$ ratio Significance A
1	Unigene71408_B	gi\|296490750\|	collagen alpha-1(III) chain	1.166	0.048	0.041	0.032
2	Unigene36652_B	gi\|148686175\|	mCG142254, partial	3.824	0.190	0.050	0.038
3	Unigene23627_B	gi\|301773364\|	PREDICTED: a-kinase anchor protein 13-like	4.674	0.233	0.050	0.041
4	Unigene50328_B	gi\|301613162\|	PREDICTED: IgGFc-binding protein-like	4.192	0.322	0.077	0.043
5	Unigene31388_B	gi\|301613160\|	PREDICTED: mucin-5AC-like	4.003	0.364	0.091	0.046
6	Unigene35953_B	gi\|283837861\|	annexin A1	1.515	0.139	0.092	0.048
7	Unigene35972_B	gi\|118091320\|	PREDICTED: similar to mucin	3.318	0.364	0.110	0.048

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