CDDP Core Primers in Sugar Beet: Selection and Variety Identification

doi:10.11924/j.issn.1000-6850.casb20200300206

Abstract

Abstract:

To study the application of CDDP primers in sugar beet breeding, we selected 12 sugar beet varieties for screening core primers from 35 CDDP primer pairs. Then, screened core primers were used to identify 43 registered varieties. The results showed that 10 of 35 CDDP primer pairs could be used as core CDDP primers for varieties identification. The bands amplified by these primers were clear, legible and high polymorphism and generally distributed from 50 to 400 bp. Among these core primers, KNOX-3 could identify the 43 registered varieties. The combination of MADS-1 and F3’H4 could also identify the 43 registered varieties. The screen of CDDP core primers could provide theoretical and technical support for beet variety identification and germplasm resources classification, and for effectively protecting the interests of farmers, breeders and sugar industry.

Key words: sugar beet, CDDP markers, primer selection, variety identification

CLC Number:

S556.3

Pi Zhi, Wu Zedong. CDDP Core Primers in Sugar Beet: Selection and Variety Identification[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 29-32.

Figures/Tables 4

References 23

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品种编号	品种名称	品种编号	品种名称	品种编号	品种名称
1	kws1231	16	KuHN1125	31	BTS705
2	kws1197	17	KuHN1387	32	BTS2730
3	Ls1210	18	KuHN5012	33	BETA176
4	kws2314	19	SV893	34	BETA468
5	ss1532	20	KuHN1178	35	BTS5950
6	kws1176	21	爱丽斯	36	BETA796
7	MA2070	22	AK3018	37	H003
8	MA3005	23	H7IM15	38	SV1375
9	MA10-4	24	PJ1	39	SR496
10	Flores	25	ZM1162	40	COFCO1001
11	MA11-8	26	VF3019	41	SV1433
12	HI0936	27	ST13929	42	HX910
13	MA3001	28	SD13829	43	IM802
14	KuHN8060	29	SD12830
15	KuHN1277	30	BTS8840

品种编号	品种名称	品种编号	品种名称	品种编号	品种名称
1	kws1231	16	KuHN1125	31	BTS705
2	kws1197	17	KuHN1387	32	BTS2730
3	Ls1210	18	KuHN5012	33	BETA176
4	kws2314	19	SV893	34	BETA468
5	ss1532	20	KuHN1178	35	BTS5950
6	kws1176	21	爱丽斯	36	BETA796
7	MA2070	22	AK3018	37	H003
8	MA3005	23	H7IM15	38	SV1375
9	MA10-4	24	PJ1	39	SR496
10	Flores	25	ZM1162	40	COFCO1001
11	MA11-8	26	VF3019	41	SV1433
12	HI0936	27	ST13929	42	HX910
13	MA3001	28	SD13829	43	IM802
14	KuHN8060	29	SD12830
15	KuHN1277	30	BTS8840

引物名称	引物序列(5'→3')
ABP1-1	ACSCCSATCCACCGC
ERF1	CACTACCGCGGSCTSCG
F3'H4	GCCCAAGCCCAASAAG
KNOX-1	AAGGGSAAGCTSCCSAAG
KNOX-2	CACTGGTGGGAGCTSCAC
KNOX-3	AAGCGSCACTGGAAGCC
MADS-1	ATGGGCCGSGGCAAGGTGC
MADS-2	ATGGGCCGSGGCAAGGTGG
WRKY-R1	GTGGTTGTGCTTGCC
WRKY-R2	GCCCTCGTASGTSGT

引物名称	引物序列(5'→3')
ABP1-1	ACSCCSATCCACCGC
ERF1	CACTACCGCGGSCTSCG
F3'H4	GCCCAAGCCCAASAAG
KNOX-1	AAGGGSAAGCTSCCSAAG
KNOX-2	CACTGGTGGGAGCTSCAC
KNOX-3	AAGCGSCACTGGAAGCC
MADS-1	ATGGGCCGSGGCAAGGTGC
MADS-2	ATGGGCCGSGGCAAGGTGG
WRKY-R1	GTGGTTGTGCTTGCC
WRKY-R2	GCCCTCGTASGTSGT