In Vitro Regeneration and Genetic Transformation System of Willow: Research Progress

doi:10.11924/j.issn.1000-6850.casb2022-0851

Abstract

Abstract:

Willow has attracted attention because of its medicinal value, ecological restoration capacity and potential as a bioenergy source. In recent years, many excellent willow varieties have been cultivated, and candidate genes related to economic traits and stress resistance have been identified in the genome of Salix. A large number of high-quality willow saplings can be obtained by establishing in vitro regeneration and genetic transformation system, and the function of candidate genes can be studied. In order to have a clear understanding of different factors affecting willow regeneration and genetic transformation, this paper reviewed the research progress of willow in vitro regeneration and genetic transformation, including the selection of optimal medium for primary culture, subculture and rooting culture of different Salix species, the selection of optimal medium, hormone and explant type for callus induction, the effects of Agrobacterium type, concentration, infection time, preculture and co-culture time on genetic transformation, and the optimal concentration of kanamycin for selecting efficiency of genetic transformation. At present, there are still some difficulties in the regeneration and genetic transformation of willow callus. In this study, further research directions of willow callus regeneration and genetic transformation were discussed to provide reference for establishing the in vitro regeneration and genetic transformation system of willow, and forming a foundation for the subsequent genetic engineering research and genetic improvement of willow.

Key words: willow, in vitro regeneration, genetic transformation

LIU Yuan, LI Jihong, QIN Guanghua, LIU Cuishuang, SUN Maotong, NIU Muge, WANG Jinnan. In Vitro Regeneration and Genetic Transformation System of Willow: Research Progress[J]. Chinese Agricultural Science Bulletin, 2023, 39(29): 32-38.

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