Abstract: The objective was to provide a scientific basis for the germinability monitoring, method establishment and safe storage time identification of maize germplasm and other crops germplasm stored in the genebank. The germination energy and germination rate of 1729 seed accessions, belonged to 8 species of different storage numbers of maize germplasm resources, were monitored after being stored in the genebank for 27-32 years. The results showed that 27-32 years after the storage, the average germination energy of the seeds was 82.66%, the average germination rate was 88.09%, and the germination rate of 86.58% accessions was above 75%, but the germination rate of 73 accessions was below 50%. The germination rate of seeds stored for 31-32 years decreased significantly, but the decrease of the average germination rate of seeds stored for 27-30 years was not remarkable. The germination rates of seeds from different origins with long-term storage for 31 years decreased differently, the native germplasm in Guangxi had higher germination rate, compared with germplasm from other original producing areas and abroad. The maize germplasm stored in different years or stored in the same year with different origins had different decreasing trends under long-term low temperature.The maize germplasm resources in long-term genebank could be safely conserved for more than 30 years.