Trichoderma longibrachiatum Strain TS-1: Isolation, Identification, Antagonism and Solid Fermentation Condition

doi:10.11924/j.issn.1000-6850.casb2020-0799

Abstract

Abstract:

To screen biocontrol fungi that can effectively inhibit common plant diseases, a strain of Trichoderma TS-1 was isolated from the rhizosphere soil of a potato continuous cropping field in Jingtai County, Gansu Province. It was identified as Trichoderma longibrachiatum based on both morphological characteristics and phylogenetic analysis of translation elongation factor 1-alpha genes. The inhibitory effect of TS-1 on six different pathogens was determined by plate confrontation culture. Wheat seedlings were inoculated with TS-1 at different concentrations, and the seed morphological indices were analyzed. The fermentation conditions were evaluated using a single-factor method to determine the spore yield. The results show that T. longibrachiatum strain TS-1 has a positive bacteriostatic effect on Fusarium solani, F. sambucinum and Botrytis cinerea, and the inhibition rate is more than 70%. Compared with the control, it could promote the growth of wheat seeds to an extent. The maximum spore production is obtained using the TS-1 culture solids under the following fermentation condition: using the main ingredients of straw and bran as 9:1, adding 10% of barley, and taking lactose and ammonium sulphate as the carbon and nitrogen source. The above experiment shows that TS-1 has excellent biocontrol potential and its culture could be further expanded.

Key words: Trichoderma longibrachiatum, identification, antagonism, growth-promoting, solid fermentation

CLC Number:

S435.131

Zhang Xiaojie, Zhou Tianwang, Wang Chunming, Guo Cheng. Trichoderma longibrachiatum Strain TS-1: Isolation, Identification, Antagonism and Solid Fermentation Condition[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 105-111.

Figures/Tables 9

References 32

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种名	菌株	寄主	国家	登录号
长枝木霉T. longibrachiatum	DAOM 166989	unknown	Canada	EU338335
长枝木霉T. longibrachiatum	GJS 01-121	Crinipellis sp	Netherlands	JN175564
长枝木霉T. longibrachiatum	CPK 3276	Pleurotus ostreatus	unknown	EU918159
长枝木霉T. longibrachiatum	CBS 816.68	unknown	unknown	AY865640
长枝木霉T. longibrachiatum	DAOM 234103	unknown	unknown	DQ125467
长枝木霉T. longibrachiatum	OY6607	unknown	unknown	FJ619248
长枝木霉T. longibrachiatum	SzMC	unknown	unknown	EU401624
长枝木霉T. longibrachiatum	SzMC IM3	unknown	unknown	EU401627
长枝木霉T. longibrachiatum	CIB T13	unknown	Colombia	EU280033

种名	菌株	寄主	国家	登录号
长枝木霉T. longibrachiatum	DAOM 166989	unknown	Canada	EU338335
长枝木霉T. longibrachiatum	GJS 01-121	Crinipellis sp	Netherlands	JN175564
长枝木霉T. longibrachiatum	CPK 3276	Pleurotus ostreatus	unknown	EU918159
长枝木霉T. longibrachiatum	CBS 816.68	unknown	unknown	AY865640
长枝木霉T. longibrachiatum	DAOM 234103	unknown	unknown	DQ125467
长枝木霉T. longibrachiatum	OY6607	unknown	unknown	FJ619248
长枝木霉T. longibrachiatum	SzMC	unknown	unknown	EU401624
长枝木霉T. longibrachiatum	SzMC IM3	unknown	unknown	EU401627
长枝木霉T. longibrachiatum	CIB T13	unknown	Colombia	EU280033

菌株	抑菌率/%
禾谷镰孢 F. graminearum	52.69 ± 0.01 e
尖孢镰孢 F. oxysporum	57.94 ± 0.01 d
拟轮枝镰孢 F. verticillioides	59.84 ± 0.01 d
接骨木镰孢 F. sambucinum	70.64 ± 0.01 ab
茄病镰孢 F. solani	72.88 ± 0.03 a
灰葡萄孢霉 Botrytis cinerea	73.81± 0.02 a

菌株	抑菌率/%
禾谷镰孢 F. graminearum	52.69 ± 0.01 e
尖孢镰孢 F. oxysporum	57.94 ± 0.01 d
拟轮枝镰孢 F. verticillioides	59.84 ± 0.01 d
接骨木镰孢 F. sambucinum	70.64 ± 0.01 ab
茄病镰孢 F. solani	72.88 ± 0.03 a
灰葡萄孢霉 Botrytis cinerea	73.81± 0.02 a

处理	发芽率	芽长	根长	根数	根冠比
CK	93.33 ±6.67 a	2.96 ±0.12 b	6.25±0.10 b	3.23±0.05 c	0.67±0. 13b
1×10⁵	100.00 ±0.00 a	3.12±0.03 b	7.13±0.16 a	3.34 ±0.13 bc	0.89 ±0. 13 ab
1×10⁶	100.00 ±0.00 a	3.59±0.04 a	6.99± 0.14 a	3.58 ±0.08 ab	0.99 ±0. 12 a
1×10⁷	96.67 ±3.33 a	3.81 ±0.14 a	7.31± 0.06 a	3.80 ±0.09a	0.94 ±0. 08 a