Identification and Characterization of Opsin Genes from Thalassodes immissaria and Gene Expression After White LED Treatment

doi:10.11924/j.issn.1000-6850.casb2021-0615

Abstract

Abstract:

Currently, lighting control technology is promoted in litchi and longan cultivation areas in China. To explore the impacts of the lighting control technology on litchi leaf looper, 2 opsin genes were cloned using RACE technology based on the transcriptome sequencing results from Thalassodes immissaria, and then expression changes of the 2 opsin genes were detected after light treatment to T. immissaria by RT-qPCR. The results showed that the open reading frames of TiLW and TiUV encoded 374 and 378 amino acids, respectively. Results of secondary structure analysis of the 2 opsin genes showed that both of them were mainly composed of α-helix and random coil and contained typical conserved domains of opsin family. The 2 opsin genes could be detected at different developmental stages of the whole development cycle, but their expression levels were different at different developmental stages. After light treatment, the expression levels of TiLW and TiUV in the head of adults were affected by different degrees. Compared with the control group, after the light treatment of 24 h, TiLW was increased by 12.96 times and decreased by 8.61 times in female and male heads, respectively, while TiUV was increased by 1.75 times and decreased by 3.10 times, respectively. The results of this study show that TiLW and TiUV are involved in the stress process of light stimulation, and light stimulation could cause changes in the expression of the two opsin genes.

Key words: Thalassodes immissaria, opsin, light stimulation, light interference technology, sequence analysis, gene expression

CLC Number:

S881.2

YAO Qiong, QUAN Linfa, XU Shu, DONG Yizhi, LI Wenjing, CHI Yanyan, CHEN Bingxu. Identification and Characterization of Opsin Genes from Thalassodes immissaria and Gene Expression After White LED Treatment[J]. Chinese Agricultural Science Bulletin, 2022, 38(16): 103-109.

Figures/Tables 6

References 28

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项目	引物名称	序列
全长扩增	TiLW-F	ACGTGGCTTCTCTGCGATCAGC
	TiLW-R	GCTGGAGACATAGCGCACAT
	TiUV-F	AGGACAGAAGACACTTATCAGC
	TiUV-R	TGGCCATACTTGCCACATCCTG
荧光定量PCR	TiLW	TCTGGCAATGGAATGGTCATCTAT
	TiLW	GCACATCATTAGGAAATCGGAGAAA
	TiUV	GAGGTTATTCTCATGTGGCTGGAA
	TiUV	CACAGGCAATGTTGGTGAATGTTA
	Tiâ-actin	AATCCCTACTTCGTGAGCGTGAA
	Tiâ-actin	GCCAAACTCTTTAGCAATGTCAGGA

项目	引物名称	序列
全长扩增	TiLW-F	ACGTGGCTTCTCTGCGATCAGC
	TiLW-R	GCTGGAGACATAGCGCACAT
	TiUV-F	AGGACAGAAGACACTTATCAGC
	TiUV-R	TGGCCATACTTGCCACATCCTG
荧光定量PCR	TiLW	TCTGGCAATGGAATGGTCATCTAT
	TiLW	GCACATCATTAGGAAATCGGAGAAA
	TiUV	GAGGTTATTCTCATGTGGCTGGAA
	TiUV	CACAGGCAATGTTGGTGAATGTTA
	Tiâ-actin	AATCCCTACTTCGTGAGCGTGAA
	Tiâ-actin	GCCAAACTCTTTAGCAATGTCAGGA

基因	CDS长度/ bp	开放阅读框长度/ bp	分子量/ kDa	等电点	亲水系数	跨膜螺旋数	α-螺旋比例/ %	β-转角比例/ %	随机卷曲比例/ %	延伸链比例/ %
TiLW	1504	1137	41.04	7.01	0.415	7	45.01	2.70	33.15	19.14
TiUV	1726	1125	41.52	5.69	-3.20	7	2.93	7.47	60.80	28.80

基因	CDS长度/ bp	开放阅读框长度/ bp	分子量/ kDa	等电点	亲水系数	跨膜螺旋数	α-螺旋比例/ %	β-转角比例/ %	随机卷曲比例/ %	延伸链比例/ %
TiLW	1504	1137	41.04	7.01	0.415	7	45.01	2.70	33.15	19.14
TiUV	1726	1125	41.52	5.69	-3.20	7	2.93	7.47	60.80	28.80