Abstract:

The study aims to optimize the tissue culture reproduction and regeneration system of Euonymus maackii, solve the problem of small multiplication coefficient when the axillary bud germination is used as the regeneration method for tissue culture. Taking the young stems of E. maackii as the materials, we sterilized them with 20% sodium hypochlorite and 0.1% mercuric chloride respectively, and set the treatment time as 8, 10 and 12 minutes, to obtain the best sterilization plan. Using E. maackii stems as the materials, by adjusting the concentration of 6-BA and NAA, the stems of E. maackii were induced to produce callus. The callus of E. maackii was used as the material, and the adventitious buds were induced by 6-BA and NAA orthogonal test. Using the adventitious buds of E. maackii as the materials, we adjusted the concentration of 6-BA and NAA to increase the proliferation coefficient of subsequent generations. Using E. maackii seedlings as the materials, we adjusted the concentration of IBA and NAA to obtain rooting seedlings of E. maackii. The results showed that: (1) the best solution for sterilizing the young stems of E. maackii was 20% sodium hypochlorite for 12 minutes, and the pollution rate was 2%; (2) the best medium for stem callus induction was 1.0 mg/L 6-BA+ 0.10 mg/L NAA, and the induction rate was 80.00%; (3) the best medium for adventitious bud induction was 0.5 mg/L 6-BA+ 0.10 mg/L NAA, and the induction rate was 91.11%; (4) the best medium for subsequent proliferation was MS+1.0 mg/L 6-BA+ 0.10 mg/L NAA, and the proliferation coefficient was 6; (5) the best medium for rooting culture was 1/2MS+IBA 0.3 mg/L, and the average number of roots was 5. The callus is induced from the budless stems of E. maackii, and the proliferation coefficient is increased to 6. The optimization of the tissue culture regeneration system of E. maackii is achieved.

Key words: Euonymus maackii, callus induction, adventitious bud, regeneration system, subsequent proliferation