Bellamya aeruginosa in Six Lakes Along the East Line of the South-to-North Water Diversion Project: Analysis of Genetic Diversity and Genetic Structure

doi:10.11924/j.issn.1000-6850.casb2022-0313

Abstract

Abstract:

In order to evaluate the impact of the South-to-North Water Diversion Project on water environment and ecology of lakes along the water conveyance route, using wild Bellamya aeruginosa in different lakes and water area (Dongping Lake, Nansi Lake, Luoma Lake, Hongze Lake, Gaoyou Lake etc.) along the east line of the project as the materials, and that in Dianchi Lake as the control, the genetic diversity and phylogenetic relationship of various populations were analyzed based on COI and 16S rRNA genes. The results showed that the haplotype diversity (0.924, 0.958), nucleotide diversity (0.04466, 0.03068) and average nucleotide diversity (27.333, 14.117) of Dianchi Lake population were much higher than those of the six populations along the east line of the South-to-North Water Diversion Project. Tajima’s neutral test results did not deviate from the neutral significantly (P>0.10), and the nucleotide pairing difference distribution map did not show an obvious single peak. The genetic distance between Dianchi Lake population and the six populations along the east line was much greater than that among the six populations. The cluster analysis showed that most individuals of the six populations along the east line of the South-to-North Water Diversion Project were mixed together, while most individuals of the Dianchi Lake population were concentrated together, mixing with other populations occasionally. The study indicates that the water diversion project has an impact on the genetic diversity and genetic structure of Bellamya aeruginosa in waters along the project route.

Key words: South-to-North Water Diversion Project, Bellamya aeruginosa, COI, 16S rRNA, genetic diversity, genetic structure

ZHAO Yan, ZHANG Chong, CUI Mengyao, LI Xian, LU Guangjin, JI Xiangshan, CHEN Hongju. Bellamya aeruginosa in Six Lakes Along the East Line of the South-to-North Water Diversion Project: Analysis of Genetic Diversity and Genetic Structure[J]. Chinese Agricultural Science Bulletin, 2023, 39(12): 54-60.

Figures/Tables 8

References 24

[1]	范强. 铜锈环棱螺用于水生态毒理学的基础研究[D]. 天津: 天津农学院, 2015.
[2]	VALDOVINOS C, MOYA C, OLMOS V, et al. The importance of water-level fluctuation for the conservation of shallow water benthic macroinvertebrates: an example in the Andean zone of Chile[J]. Biodiversity & conservation, 2007, 16(11):3095-3109.
[3]	AROVIITA J, HMLINEN H. The impact of water-level regulation on littoral macroinvertebrate assemblages in boreal lakes[J]. Hydrobiologia, 2008, 613(1):45-56. doi: 10.1007/s10750-008-9471-4 URL
[4]	Yu Z D, WANG H, MIAO M S, et al. Long-term monitoring of community succession in impoundment lake: responses of macroinvertebrate to south-to-north water diversion project[J]. Ecological indicators, 2020, 118:106734. doi: 10.1016/j.ecolind.2020.106734 URL
[5]	MENEZES S, BAIRD D J, SOARES A. Beyond taxonomy: a review of macroinvertebrate trait-based community descriptors as tools for freshwater biomonitoring[J]. Journal of applied ecology, 2010, 47(4):711-719. doi: 10.1111/jpe.2010.47.issue-4 URL
[6]	CHEN J, SHEN H, ZHU T, et al. Macroinvertebrate community structure and its relationships with environmental factors in the Gaoyou lake, eastern China[J]. Acta ecologica sinica, 2021, 41(16):6486-6493.
[7]	张亚平, 施立明. 动物线粒体DNA多态性的研究概况[J]. 动物学研究, 1992, 13(3):289-298.
[8]	彭居俐, 王绪桢, 何舜平. DNA条形码技术的研究进展及其应用[J]. 水生生物学报, 2008, 32(6):916-919.
[9]	翁朝红, 谢仰杰, 肖志群, 等. 线粒体COI和16S rRNA片段确定近江蛏和缢蛏属的分类地位[J]. 水生生物学报, 2013, 37(4):684-690.
[10]	李宏俊, 张晶晶, 袁秀堂, 等. 利用线粒体COI和微卫星标记分析文蛤7个地理群体的遗传变异[J]. 生态学报, 2016, 36(2):499-507.
[11]	于贞贞. 异齿亚纲贝类DNA条形码与系统发生学研究[D]. 青岛: 中国海洋大学, 2014.
[12]	焦明超. 环棱螺属部分种类DNA条形码研究及其系统发育分析[D]. 南昌: 南昌大学, 2013.
[13]	陈思. 南四湖环棱螺的形态分类及分子系统发育研究[D]. 曲阜: 曲阜师范大学, 2013.
[14]	GU Q H, HUSEMANN M, DING B, et al. Population genetic structure of Bellamya aeruginosa (Mollusca: Gastropoda: Viviparidae) in China: weak divergence across large geographic distances[J]. Ecology & evolution, 2015, 5(21):4906-4919.
[15]	符蓉. 鄱阳湖及长三角部分水域常见淡水软体动物物种条形码及图谱研究[D]. 南京: 南京农业大学, 2017.
[16]	WAS G, BOWEN B W. Shallow population histories in deep evolutionary lineages of marine fishes: Insights from sardines and anchovies and lessons for conservation[J]. Journal of heredity, 1998, 89(5):415-426. doi: 10.1093/jhered/89.5.415 URL
[17]	高路, 高艳菊, 何佳, 等. 人为活动干扰下滇池湖泊稳态过程浅析[J]. 中央民族大学学报:自然科学版, 2019, 28(4):77-82.
[18]	KORNELIUSSEN T S, MOLTKE I, ALBRECHTSEN A, et al. Calculation of Tajima's D and other neutrality test statistics from low depth next-generation sequencing data[J]. BMC bioinformatics, 2013, 14(1):1-14. doi: 10.1186/1471-2105-14-1
[19]	顾钱洪. 中国不同地理群体环棱螺遗传多样性和分类阶元的研究[D]. 武汉: 华中农业大学, 2013.
[20]	POSADA D, CRANDALL K A. Selecting the best-fit model of nucleotide substitution[J]. Systematic biology, 2001, 50(4):580-601. pmid: 12116655
[21]	李秀启, 刘峰, 冷春梅. 基于线粒体DNA控制区的南四湖湖鲚(Coilia ectenes taihuensis)群体遗传结构和种群扩散[J]. 湖泊科学, 2015, 27(4):686-692.
[22]	GUO C, CHEN Y, GOZLAN R E, et al. Patterns of fish communities and water quality in impounded lakes of China's south-to-north water diversion project[J]. Science of the total environment, 2020, 713:136515. doi: 10.1016/j.scitotenv.2020.136515 URL
[23]	GITTENBERGER E, GROENENBERG D, KOKSHOORN B, et al. Biogeography: molecular trails from hitch-hiking snails[J]. Nature, 2006, 439(7075):409. doi: 10.1038/439409a
[24]	CASPER H A, LEEUWEN V, GERARD VAN DER V, BART VAN L, et al. Experimental quantification of long distance dispersal potential of aquatic snails in the gut of migratory birds[J]. Plos one, 2012, 7(3):1-7.

群体	个体数	采样区域	采样时间
东平湖DP	50	35.99°N，119.19°E	2020.09
微山湖WS	50	34.65°N，117.27°E	2020.09
骆马湖LM	50	34.06°N，118.17°E	2020.10
洪泽湖HZ	50	33.07°N，118.74°E	2020.10
高邮湖GY	50	32.71°N，119.31°E	2020.10
长江CJ	50	32.23°N，119.39°E	2020.11
滇池DC	50	24.80°N，102.70°E	2020.11

群体	个体数	采样区域	采样时间
东平湖DP	50	35.99°N，119.19°E	2020.09
微山湖WS	50	34.65°N，117.27°E	2020.09
骆马湖LM	50	34.06°N，118.17°E	2020.10
洪泽湖HZ	50	33.07°N，118.74°E	2020.10
高邮湖GY	50	32.71°N，119.31°E	2020.10
长江CJ	50	32.23°N，119.39°E	2020.11
滇池DC	50	24.80°N，102.70°E	2020.11

群体	单倍型数量H	单倍型多样性Hd	多态位点数S	平均核苷酸差异数K	核苷酸多样性Pi
东平湖DP	6	0.579	15	3.453	0.00563
微山湖WS	8	0.700	16	5.347	0.00872
骆马湖LM	8	0.805	16	3.621	0.00591
洪泽湖HZ	9	0.889	40	10.642	0.01736
高邮湖GY	7	0.711	18	4.747	0.00828
长江CJ	4	0.647	35	7.137	0.01164
滇池DC	11	0.924	79	27.333	0.04466

群体	单倍型数量H	单倍型多样性Hd	多态位点数S	平均核苷酸差异数K	核苷酸多样性Pi
东平湖DP	6	0.579	15	3.453	0.00563
微山湖WS	8	0.700	16	5.347	0.00872
骆马湖LM	8	0.805	16	3.621	0.00591
洪泽湖HZ	9	0.889	40	10.642	0.01736
高邮湖GY	7	0.711	18	4.747	0.00828
长江CJ	4	0.647	35	7.137	0.01164
滇池DC	11	0.924	79	27.333	0.04466

群体	单倍型数量H	单倍型多样性Hd	多态位点数S	平均核苷酸差异数K	核苷酸多样性Pi
东平湖DP	4	0.600	9	1.700	0.00368
微山湖WS	6	0.747	5	1.216	0.00263
骆马湖LM	10	0.800	23	3.574	0.00774
洪泽湖HZ	9	0.826	25	4.884	0.01057
高邮湖GY	6	0.516	7	0.789	0.00171
长江CJ	5	0.568	7	1.468	0.00328
滇池DC	15	0.958	52	14.117	0.03068