Preliminary Establishment of Tissue Culture Regeneration System for Begonia benariensis ‘BIG’

doi:10.11924/j.issn.1000-6850.casb2022-0944

Abstract

Abstract:

The Begonia benariensis ‘BIG’ has high ornamental and economic value, and has broad application prospects. However, the varieties of B. benariensis ‘BIG’ grown in China rely on foreign imports. MS and 1/2MS were used as basic media, and combined with different plant hormones respectively to study the effects of combination media on adventitious bud differentiation, regeneration and rooting of B. benariensis, so as to establish a rapid propagation system for B. benariensis, and to solve the germplasm resource dilemma in production. The results showed that the optimal medium for adventitious bud differentiation was MS+1 mg/L 6-BA+ 0.1 mg/L 2.4-D+ 0.2 mg/L NAA. The optimal medium for adventitious bud subculture was MS+1 mg/L 6-BA+ 0.1 mg/L NAA. The optimal medium for rooting was 1/2MS+ 1.0 mg/L NAA. The experiment established a tissue culture regeneration system for B. benariensis ‘BIG’, which provided a theoretical basis for large scale culture and propagation.

Key words: Begonia benariensis, tissue culture, callus differentiation, proliferation coefficient, rooting culture, medium formulation

CHEN Caixia, HUANG Min, ZHAO Yi, CAO Chuanqu, WANG Jisheng, SHI Zhihua, WANG Gefeng, GAO Junshan. Preliminary Establishment of Tissue Culture Regeneration System for Begonia benariensis ‘BIG’[J]. Chinese Agricultural Science Bulletin, 2023, 39(34): 86-91.

Figures/Tables 9

References 27

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编号	基础培养基	6-BA/(mg/L)	2,4-D/(mg/L)	NAA/(mg/L)
B₁	MS	1	0	0
B₂	MS	2	0	0
B₃	MS	2	0.1	0
B₄	MS	2	0.5	0
B₅	MS	1	0.1	0
B₆	MS	1	0.5	0
B₇	MS	1	0	0.2
B₈	MS	1	0.1	0.2
B₉	MS	1	0.5	0.5

编号	基础培养基	6-BA/(mg/L)	2,4-D/(mg/L)	NAA/(mg/L)
B₁	MS	1	0	0
B₂	MS	2	0	0
B₃	MS	2	0.1	0
B₄	MS	2	0.5	0
B₅	MS	1	0.1	0
B₆	MS	1	0.5	0
B₇	MS	1	0	0.2
B₈	MS	1	0.1	0.2
B₉	MS	1	0.5	0.5

编号	基础培养基	6-BA/(mg/L)	NAA/(mg/L)
P₁	MS	0.5	0
P₂	MS	1	0.1
P₃	MS	1	0.3
P₄	MS	1	0.5
P₅	MS	2	0
P₆	MS	2	0.1
P₇	MS	2	0.3
P₈	MS	2	0.5
P₉	MS	3	0

编号	基础培养基	6-BA/(mg/L)	NAA/(mg/L)
P₁	MS	0.5	0
P₂	MS	1	0.1
P₃	MS	1	0.3
P₄	MS	1	0.5
P₅	MS	2	0
P₆	MS	2	0.1
P₇	MS	2	0.3
P₈	MS	2	0.5
P₉	MS	3	0

编号	基础培养基	IBA/(mg/L)	NAA/(mg/L)
R₁	MS	0.5	0
R₂	MS	1	0.1
R₃	1/2MS	1	0.3
R₄	1/2MS	1	0.5
R₅	MS	2	0
R₆	MS	2	0.1
R₇	1/2MS	2	0.3
R₈	1/2MS	2	0.5