Isolation and Identification of Endophytic Bacteria in Catalpa bungei Tissue Culture Seedling and Its Functional Characteristic Analysis

doi:10.11924/j.issn.1000-6850.casb2024-0093

Abstract

Abstract:

This study aimed to explore the endophytic bacterial resources of Catalpa bungei tissue culture seedlings, to develop agricultural microorganisms with application potential, promoting the rapid growth and propagation of tissue culture seedlings. The endophytic bacteria were isolated and identified from C. bungei tissue culture seedlings by selective medium in this paper. The nitrogen content in the culture solution was determined to evaluate the nitrogen fixing effect after preliminary screening with Ashby solid medium, and the phosphorus solubilization effect was evaluated using phosphorus content in the culture solution after preliminary screening with Nbrip solid medium, then the inhibitory effect of endophytic bacteria was measured with Alternaria alternate and Fomitopsis semiolaccatus as test objects. 24 strains of endophytic bacteria were isolated from the seedling of C. bungei, and they were identified as Rhizobium zeae by morphological and 16S rRNA sequence analysis. The results indicated that the nitrogen fixing capacity of strain NF6 was best as its nitrogen concentration in the Ashby liquid medium reached 111.97 mg/L, and strain NF11 had the best phosphorus solubilization effect due to the phosphorus concentration in Nbrip culture medium reached 1007.95 mg/L. Furthermore, most of the endophytic R. zeae had inhibitory effects on the tested pathogens, and more than 20% of inhibitory rates against A. alternate and F. semiolaccatus respectively had obtained for the 6 and 2 strains of endophytic R. zeae. In this study, the resources of endophytic bacteria with the potentials of promoting growth and resistance to pathogens were obtained, which provided theoretical basis for the potential application of endophytic bacteria in tissue culture seedlings.

Key words: Catalpa bungei, tissue culture seedling, endophyte, isolation and identification, functional characteristics, nitrogen fixation, phosphorus solubilization

WANG Luyao, GUO Zeliang, CHEN Weiwei, MU Yanjuan, XIE Xiaoman, TONG Boqiang, CHENG Shiwei, LU Yizeng. Isolation and Identification of Endophytic Bacteria in Catalpa bungei Tissue Culture Seedling and Its Functional Characteristic Analysis[J]. Chinese Agricultural Science Bulletin, 2024, 40(31): 7-13.

Figures/Tables 7

References 30

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菌株序号	菌落直径/mm	菌株序号	菌落直径/mm
NF1	5.45±0.02	NF13	4.05±0.25
NF2	6.00±0.08	NF14	5.07±0.07
NF3	5.38±0.08	NF15	3.88±0.24
NF4	5.63±0.01	NF16	4.05±0.03
NF5	5.37±0.01	NF17	5.25±0.03
NF6	7.00±0.07	NF18	5.39±0.07
NF7	5.75±0.15	NF19	4.88±0.05
NF8	5.13±0.10	NF20	6.32±0.19
NF9	3.38±0.05	NF21	7.63±0.15
NF10	4.87±0.05	NF22	6.87±0.28
NF11	3.75±0.12	NF23	6.13±0.35
NF12	3.38±0.11	NF24	7.50±0.09

菌株序号	菌落直径/mm	菌株序号	菌落直径/mm
NF1	5.45±0.02	NF13	4.05±0.25
NF2	6.00±0.08	NF14	5.07±0.07
NF3	5.38±0.08	NF15	3.88±0.24
NF4	5.63±0.01	NF16	4.05±0.03
NF5	5.37±0.01	NF17	5.25±0.03
NF6	7.00±0.07	NF18	5.39±0.07
NF7	5.75±0.15	NF19	4.88±0.05
NF8	5.13±0.10	NF20	6.32±0.19
NF9	3.38±0.05	NF21	7.63±0.15
NF10	4.87±0.05	NF22	6.87±0.28
NF11	3.75±0.12	NF23	6.13±0.35
NF12	3.38±0.11	NF24	7.50±0.09

菌株序号	解磷圈与菌落直径比	菌株序号	解磷圈与菌落直径比
NF1	—	NF13	2.25±0.12
NF2	2.42±0.04	NF14	2.81±0.53
NF3	2.21±0.58	NF15	0.82±0.16
NF4	2.25±0.23	NF16	1.74±0.10
NF5	1.77±0.06	NF17	1.17±0.02
NF6	2.02±0.33	NF18	2.28±0.48
NF7	1.80±0.52	NF19	1.94±0.08
NF8	1.28±0.12	NF20	2.31±0.81
NF9	1.72±0.43	NF21	2.52±0.88
NF10	1.72±0.12	NF22	2.70±0.17
NF11	1.89±0.41	NF23	2.20±0.37
NF12	2.23±0.65	NF24	1.82±0.01

菌株序号	解磷圈与菌落直径比	菌株序号	解磷圈与菌落直径比
NF1	—	NF13	2.25±0.12
NF2	2.42±0.04	NF14	2.81±0.53
NF3	2.21±0.58	NF15	0.82±0.16
NF4	2.25±0.23	NF16	1.74±0.10
NF5	1.77±0.06	NF17	1.17±0.02
NF6	2.02±0.33	NF18	2.28±0.48
NF7	1.80±0.52	NF19	1.94±0.08
NF8	1.28±0.12	NF20	2.31±0.81
NF9	1.72±0.43	NF21	2.52±0.88
NF10	1.72±0.12	NF22	2.70±0.17
NF11	1.89±0.41	NF23	2.20±0.37
NF12	2.23±0.65	NF24	1.82±0.01

菌株序号	链格孢菌	葱柄锈菌
NF1	11.40±0.72	8.89±0.39
NF2	19.30±1.21	11.11±0.24
NF3	13.16±0.53	7.78±0.31
NF4	21.05±1.48	11.11±0.67
NF5	22.81±0.82	2.22±0.12
NF6	19.30±1.66	24.44±2.15
NF7	9.65±0.19	6.67±0.41
NF8	12.28±0.28	11.11±0.81
NF9	23.68±1.49	12.22±0.56
NF10	18.42±1.94	20.00±2.38
NF11	11.40±049	8.89±0.60
NF12	21.05±2.28	6.67±0.29
NF13	21.05±1.55	18.89±1.09
NF14	18.42±6.17	15.56±1.44
NF15	2.63±0.69	4.44±0.67
NF16	17.54±2.57	10.00±2.55
NF17	14.04±1.41	17.78±3.09
NF18	10.53±1.77	8.89±1.23
NF19	12.28±0.89	—
NF20	8.77±1.46	—
NF21	8.77±2.05	3.33±0.74
NF22	21.05±3.19	15.56±2.69
NF23	14.04±1.82	11.11±2.71
NF24	—	—