Characterization of Arabidopsis thaliana 2-methyl-6-phytyl-1,4-benzoquinol Methyltransferase (MPBQ MT) Gene Promoter in Transgenic Tobacoo

Abstract

Abstract: The expression pattern of the promoter of Arabidopsis gene 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT) in transgenic tobacco was studied according to the analysis of GUS reporter gene. The promoter was fused with GUS reporter gene and this expression cassette was introduced into wild tobacco by Agrobacterium-mediated transformation. The expression pattern of the promoter was analyzed by GUS staining and quantitative fluorometric GUS assay. The GUS staining wasn’t observed in roots and seeds of transgenic tobacco, which was visualized in stems and leaves. The GUS expression was the highest in leaves, which was about 4.7~10.9 times of stems. MPBQ MT promoter was expressed preferentially in the green tissues of tobacco.

CLC Number:

Q786

Liu Bin,, Wang Lei, Zhang Wei. Characterization of Arabidopsis thaliana 2-methyl-6-phytyl-1,4-benzoquinol Methyltransferase (MPBQ MT) Gene Promoter in Transgenic Tobacoo[J]. Chinese Agricultural Science Bulletin, 2007, 23(4): 33-33.

References

[1] Sattler S E, Cheng Z, DellaPenna D. From Arabidopsis to agriculture: Engineering improved vitamin E content in soybean . Trends Plant Sci. 2004,9:365-367.
[2] Dean DellaPenna, Barry J Pogson. Vitamin Synthesis in Plants: tocopherols and Carotenoids.Annu Rev Plant Biol. 2006, 57:711-38.
[3] Shintani D K, Cheng Z, DellaPenna D. The role of 2-methyl-6-phytylbenzoquinone methyltranseferase in determining tocopherol composition in synechocystis sp. PCC6803. FEBS Lett. 2002, 511:1-5.
[4] Porfirova S, Bergmuller E, Tropf S, et al. Isolation of an Arabidopsis mutant lacking Vitamin E and identification of a cyclase essential for all tocoppherol biosynthesis. Proc Natl Acad Sci USA. 2002,99:12495-12500.
[5] Shintani D, Dellapenna D. Elevating the vitamin E content of plants through metabolic engineering. Science. 1998,282:2098-2100.
[6] Eva Collakova, Dean DellaPenna. The Role of Homogentisate Phytyltransferase and Other Thcopherol Pathway Enzymes in the Regulation of Tocopherol Synthesis during Abiotic Stress. Plant Physiology. 2003,133:930-940.
[7] Alison L, Van Eenennaam, Kim Lincoln, et al. Enginerring Vitamin E Content: From Arabidopsis Mutant to Soy Oil . The Plant Cell. 2003,15:3007-3019.
[8] National Research Council(U.S), Food and Nutrition Board. NAS-NRC Recommended Dietary Allowances. 10th ed. Washington, DC: National Academy Press, 1989.284.
[9] Buring J E, Hennekens C H. Antioxidant vitamins and cardiovascular disease. Nutr Rev. 1997,55:S53-S60.
[10] Tangney C C. Vitamin E and cardiovascular disease. Nutr Today. 1997,32:13-22.
[11] Grusak M A, Dellapenna D. Improving the nutrient compositon of plants to enhance human nutrition and health. Annu Rev Plant Physiol Plant Mol Biol. 1999,50:133-161.
[12] Eitenmiller R R. Vitamin E content of fats and oils: Nutritional implications. Food Technol. 1997,51:78-81.
[13] Tan B. Palm carotenoids, tocopherols and ocotrienols. J AM Oil Chem Soc.1989,66:770-776.
[14] Kanwischer M, Porfirova S, Bergmāller E, et al. Alterations in tocopherol cyclase activity in transgenic and mutant plants of Arabidopsis affect tocopherol content, tocopherol composition, and oxidative stress. Plant Physiol, 2005,137:713-723.
[15] Sergi Munne-Bosch. The role of α-tocophherol in plant stress tolerance.Journal of Plant Physiology. 2005,162:743-748.
[16] Sattler S E, Gilliland L U, Magallanes-Lundback M, ed al. Vitamin E is essential for seed longevity and for preventing lipid peroxidation during germination. Plant Cell. 2004,16:1419-1432.
[17] 周建,王磊,杜进民,等.拟南芥γ-生育酚甲基转移酶（γ-TMT）启动子的分离及表达特性分析. 生物工程学报. 2006,22:835-839.
[18] 朱永兴,王磊,张兰,等.拟南芥尿黑酸叶绿醇转移酶（HPT）基因启动子的分离及表达特性分析.作物学报,2007,33(4):554-559.
[19] Munne-Bosch S, Falk J. New insights into the function of tocopherols in plants. Planta,2004,218:323-326.