Abstract: 【Objective】 To establish the in vitro culture and expense methods of sika deer antler chondrocytes for the investigation of antler regeneration study and analyze the biological character of cultured antler chondrocytes. 【Method】 By the techniques of cell culture in vitro, the culture in vitro of the antler chondrocytes were performed. The cells growth was checked up by applying the ways of histology, MTT assay and immunohistochemistry. 【Results】 The antler chondrocytes growth rapidly in plastic cell cultural board and chondrocytes l population demonstrates extensive proliferation and retains the capacity from original passage to passage 4. The original cells growth was faster than the passage. The antler chondrocytes were triangle, polygon and other shape. Immunochemistry results showed that the cultured cells were positive for collagen IIand . dDyeing of toluidine blue was positivet. 【Conclusion】 The results showed that the chondrocytes of isolated antler presented proliferous ability in culture by our method. Chondrocytes can be cultured and extensively proliferate and retain biological feature for at least for 4 passages. The methods of antler chondrocytes culture can be used for the related continued research.