Cloning, Expression and Autophosphorylation of 5 Rice Protein Kinases

Abstract

Abstract: It has been demonstrated that protein kinases play important roles in almost all biological process, e.g. growth and development, regulation of expression, stress responses and signal transduction. The finished rice genomic sequence indicated that at least 1500 protein kinases exist in rice genome. In the current study, five rice kinases has been amplified based their full-length cDNA sequences, their corresponding proteins have been expressed and purified in yeast, the accession number are NM-189878, NM-194133, NM-190508, NM-197522 and NM-197571 respectively. NM-194133 has strong autophosphorylation activity. Based on sequence similarity, the deduced functions of these kinases are biosynthesis regulation of carbon metabolism, seed embryogenesis, cell division regulation, response to biotic/abiotic stimulation etc. This work is helpful for in vitro enzyme assay, protein-protein interaction analysis, and provides an efficient system for high throughput cloning and expression of rice genes.

CLC Number:

Q786

Liu Qian,, Sun Jian, Li LiYun, Liu LiJuan, Xu Zhouda, Wang HaiJiao, Jin MiaoJing, Liu GuoZhen. Cloning, Expression and Autophosphorylation of 5 Rice Protein Kinases[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 83-83.

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