Abstract: 【OBJECTIVE】 In order to establish the AFLP technique system in sugarcane; 【METHOD】 the hybrid parents CP84-1198 and Ke5 and correspondingly the resistance pool and susceptible pool were taken as plant materials and the AFLP(amplified fragment length polymorphism)-sliver stained experiment system was constructed accordingly. Moreover, the due system was used to analyze the drought-resistance character using the cDNA from E.arundinaceus as materials and a DD (differentially display) analysis method, which can also be named as cDNA-AFLP, was established. 【RESULTS】 During the experiment, four pairs of primers which can amplify differentially displayed bands between the resistant parent and susceptible parent, were selected. Among them, one pair of primer was found to produce favorable differentially displayed bands between the control and drought-menace E. arundinaceus. At the same time, both a quick method of silver staining of PAGE and a feasible gel conservation method were set up. And the recovery protocol of band on PAGE was also proved to be convenient and efficient. 【CONCLUSION】 In conclusion, the AFLP technique system in sugarcane analysis has been established in this paper.