Short-Spike Cutting Propagation Techniques of Tea Tree in Northern Tea Area

doi:10.11924/j.issn.1000-6850.casb2020-0337

Abstract

Abstract:

A cutting propagation suitable for tea trees in northern tea areas is proposed to provide references for the selection and promotion of cold-resistant tea varieties. An orthogonal experiment of four factors by three levels [L₉(3⁴)] was applied to studying the effects of factors such as hormone type, cutting state, dip concentration and time on tea seedling development and cutting survival rate of ‘Zhongcha 108’. The results show that: the best combination is A₁B₃C₃D₃, in which, the cuttings are soaked in clear water and dip in 200 mg/L IBA for 30 min before cutting, and the survival rate is 81.3%; from primary to secondary, the effects of the 4 cutting factors are hormones type, cutting processing method, hormone dip time and hormone concentration. Under the treatment of different hormone types, the aboveground development data of tea seedlings show that IBA+NAA treatment is beneficial to the increase of leaf number and dry weight; the root development phenotype of tea seedlings indicates that the root composition under the IBA+NAA treatment is reasonable, which is conducive to the coordinated growth of first-level lateral roots, extended roots and absorption roots in the root system; the analysis of root system development indicators show that IBA+NAA treatment could maximize the growth of total root length, total surface area, root volume, number of root tips and biomass of the root system. The optimal cutting combination is: the cuttings are soaked in clean water immediately after beveling, and then planted into the matrix after dipping 30 min in hormone combination IBA (200 mg/L) + NAA (200 mg/L). Under the optimal combination of cuttings, the cutting propagation characteristics of 27 tea tree varieties are quite different. The cutting survival rate is 91.1% at the highest, 12.17% at the lowest, and 11 tea varieties have the survival rate higher than 80%. The research results have strong applicability in northern tea tree cuttings, and can provide technical support for the selection and promotion of tea varieties.

Key words: northern tea areas, tea tree, cold-resistant tea varieties, clonal tea tree varieties, ‘Zhongcha 108’, cuttings, hormones, survival rate, variety specificity

CLC Number:

S571.1

Ren Zhihong, Wu Huanhuan, Xiao Wenmin, Zhang Hong, Sun Haiwei, Dou Ling, Wang Zhaoxiang. Short-Spike Cutting Propagation Techniques of Tea Tree in Northern Tea Area[J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 43-50.

Figures/Tables 8

References 28

[1]	虞富莲. 对加快茶树无性系良种推广的看法[J]. 中国茶叶, 2005(4):1
[2]	陈宗懋, 杨亚军. 中国茶经[M]. 上海: 上海文化出版社, 2011: 567.
[3]	林更生. 铁观音短穗扦插谁发明[J]. 福建茶业, 2005(2):35-36.
[4]	姚永宏, 吴全, 李忠林, 等. 茶树插穗生根过程中内源激素动态变化[J]. 西南农业大学学报, 2005(5):795-798.
[5]	程徽儿, 杨维时. 茶枝梢头扦插育苗的效果[J]. 茶叶科学, 1995,15(1):77-78.
[6]	周祥明, 武秀凤, 王辉琼. 无性系良种茶扦插育苗技术[J]. 江苏农业科学, 2004(3):66-77.
[7]	中国农业科学院茶叶研究所, 云南省普洱茶树良种繁育场, 福建省农业科学院茶叶研究所, 等. NY/T 2019—2011,茶树短穗扦插技术规程[S]. 北京: 中国标准出版社, 2011.
[8]	陈宗懋. 新时代中国茶产业的创新与发展[J]. 农学学报, 2018,8(1):80-84.
[9]	吴淑平, 吕立哲, 蒋双丰. 茶树扦插繁殖的原理及研究进展[J]. 中国茶叶, 2010,32(7):13-14.
[10]	吴淑平, 吕立哲, 郑杰, 等. 茶树短穗扦插成活率的影响因素探析[J]. 河南农业科学, 2014,43(10):34-37
[11]	段继华, 雷雨, 黄飞毅, 等. 不同茶树品种秋、冬扦插效果比较[J]. 茶叶通讯, 2018,45(3):44-47.
[12]	杨云丹, 王世斌. 茶树硬枝扦插研究[J]. 安徽农业科学, 2014,42(1):31-34.
[13]	邓惠静. 对影响扦插成活诸因素的分析[J]. 本溪冶金高等专科学校学报, 2003,5(2):11-13.
[14]	刘诗贤, 刘腾飞. 不同处理对北方茶树扦插成活率的研究[J]. 茶叶通讯, 2015,42(3):25-28.
[15]	胡建辉, 梁莉莉, 丁兆堂, 等. 南繁北育茶树扦插繁育方法[P]. 中国专利,CN105580689A, 2016-05-18.
[16]	王书胜, 单文, 张乐华, 等. 基质和IBA浓度对云锦杜鹃扦插生根的影响[J]. 林业科学, 2015,51(9):165-172.
[17]	王雪萍, 龚自明. 激素处理茶树扦插生根效果的综合评价[J]. 分子植物育种, 2019,17(13):4495-4500.
[18]	Husen A. Change of soluble sugars and enzymatic activities during adventitious rooting in cutting of Grewia optiva as affected by age of donor plants and auxin treatments[J]. J Plant Physiol, 2012,7(1):1-6.
[19]	陈加利, 云勇, 杨立荣, 等. 基于正交试验分析不同外源激素对油茶插穗生根的影响[J]. 分子植物育种 2018, 16(10):3430-3440.
[20]	方泽基. 茶树良种特性及其扦插繁殖技术[J]. 现代农业科技, 2013(16):26-27.
[21]	李慧, 聂枞宁, 唐茜, 等. 川西茶区高密高效扦插技术的主要影响因素分析[J]. 河南农业科学, 2016,45(5):45-51.
[22]	梁月荣, 刘祖生, 庄晚芳. 茶树插穗发根的解剖学和生物化学研究[J]. 茶叶科学, 1985(1):19-28.
[23]	Almehdi A A, Parfitt D E, Chan H. Propagation of Pistachio rootstock by rooted stem cuttings[J]. Scientia Horticulturae, 2002,96(1/2/3/4):359-363. doi: 10.1016/S0304-4238(02)00061-4 URL
[24]	郝振梅. 影响扦插育苗成活的因素[J]. 内蒙古农业科技, 2013(3):133-134.
[25]	燕丽萍, 王因花, 任飞, 等. 元宝枫嫩枝扦插快速繁育技术研究[J]. 中国农学通报, 2020,36(19):55-61.
[26]	贾志远, 葛晓敏, 唐罗忠. 木本植物扦插繁殖及其影响因素[J]. 世界林业研究, 2015,28(2):36-41.
[27]	张亚莲, 常硕其, 傅海平, 等. 茶树品种、土壤营养和扦插效果的关系[J]. 茶叶通讯, 2009,36(4):3-6.
[28]	胡惜丽, 徐可新, 朱利雄. 中黄2号茶树短穗扦插优质育苗技术[J]. 中国茶叶, 2020,42(6):46-48.

水平	因素
水平	激素种类	插穗处理方式	激素浓度/(mg/L)	浸蘸时间
1	IBA	正常处理	50	5 s
2	NAA	萎蔫处理	100	5 min
3	IBA+NAA	泡清水处理	200	0.5 h

水平	因素
水平	激素种类	插穗处理方式	激素浓度/(mg/L)	浸蘸时间
1	IBA	正常处理	50	5 s
2	NAA	萎蔫处理	100	5 min
3	IBA+NAA	泡清水处理	200	0.5 h

序号	A.激素种类	B.插穗处理方式	C.激素浓度	D.浸蘸时间	成活率/%
1	1	1	1	1	63.3
2	1	2	2	2	63.3
3	1	3	3	3	81.3
4	2	1	2	3	24.5
5	2	2	3	1	33.4
6	2	3	1	2	54.6
7	3	1	3	2	66.7
8	3	2	1	3	23.3
9	3	3	2	1	76.6
K₁	207.9	154.5	141.2	173.3
K₂	112.5	120	164.4	184.6
K₃	166.6	212.5	181.4	129.1
k₁	69.30	51.50	47.07	57.77
k₂	37.50	40.00	54.80	61.53
k₃	55.53	70.83	60.47	43.03
R	31.80	30.83	13.40	18.50

序号	A.激素种类	B.插穗处理方式	C.激素浓度	D.浸蘸时间	成活率/%
1	1	1	1	1	63.3
2	1	2	2	2	63.3
3	1	3	3	3	81.3
4	2	1	2	3	24.5
5	2	2	3	1	33.4
6	2	3	1	2	54.6
7	3	1	3	2	66.7
8	3	2	1	3	23.3
9	3	3	2	1	76.6
K₁	207.9	154.5	141.2	173.3
K₂	112.5	120	164.4	184.6
K₃	166.6	212.5	181.4	129.1
k₁	69.30	51.50	47.07	57.77
k₂	37.50	40.00	54.80	61.53
k₃	55.53	70.83	60.47	43.03
R	31.80	30.83	13.40	18.50

激素种类	叶片数	新梢长/cm	新梢径粗/cm	地上部干重/g	隶属函数
IBA	9±2.2039ab	20.44±5.3114a	0.2173±0.0485a	0.6911±0.1586a	2.53
NAA	7.2±2.3664b	14.9333±3.7506b	0.2265±0.0524a	0.5515±0.1470b	0.70
IBA+NAA	10.1333±3.563a	20.2667±4.6937a	0.2305±0.0516a	0.7036±0.1754a	3.97