Abstract: In order to analyze the detoxification effect of the spent mushroom substrate acidrolysis solution, in this paper the concentrations of the reducing sugar, the sulfuric acid root ion, the acetic acid and the furfural before and after detoxification were measured. The results showed that the reducing sugar concentration is reduced to 3.82 g/L from 6.22 g/L; Sulfuric acid root concentration is reduced to 18.90 g/L from 1.91 g/L, most sulfuric acid root ion has been removed; Acetic acid concentration are 2.13 g/L and 1.97 g/L, reduce is not obvious; Furfural concentration are 0.734 g/L and 0.124 g/L, furfural concentration was reduced by 412.52%; It obtained 0% of alcohol using not detoxified acidrolysis solution , 0.58% (w/v) of alcohol using detoxified; The detoxification effect is good obvious. The conclusion for alcoholic production study using spent mushroom substrate provides a theoretical basis.