Cloning and Sequence Analysis of Coat Protein Gene of Sweet Potato Virus G

Abstract

Abstract: Two cDNA fragments (SPVG-CPSC1, SPVG-CPSC2) with about 700bp were cloned from sweet potato (Ipomoea batats (L.) Lam.) which were suspiciously infected with sweet potato virus by reverse transcription-polymerase chain reaction（RT-PCR）method using a pair of specific primers which were designed based on the conserved region of the coat protein (CP) genes of sweet potato virus G (SPVG). PCR product was cloned into pMD18-T vector, the linked DNA was transformed into Escherichia coli JM109 and the positive clones were sequenced and analyzed. The results showed that the two cloned fragments were highly identical with known CP genes of SPVG. There was the highest identification between the Egypt 1 isolate and the two clones, with homology of the nucleotide and deduced amino acid sequences of 98.7% and 98.7% for SPVG-CPSC1, 89.1% and 94.8% for SPVG-PSC2, respectively and the lowest identification between the China isolate CH2 and the two clones, with homology of the nucleotide and deduced amino acid sequences of 87.4% and 96.6% for SPVG-CPSC1, 87.0% and 94.8% for SPVG-CPSC2, respectively. While the homology of nucleotide and deduced amino acid sequences between two cloned fragments were 89.5% and 96.1%, respectively, which indicated that the CP gene of SPVG had some polymorphism.

CLC Number:

Gu Yinghong, Tang Haoru, Zhang Yizheng. Cloning and Sequence Analysis of Coat Protein Gene of Sweet Potato Virus G[J]. Chinese Agricultural Science Bulletin, 2006, 22(9): 50-50.

References

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