Abstract: China has rich resources of Rubus L. In recent years, a deep research of Rubus L. abroad has reached good result due to the development and improvement of research methods in the field of molecular biology; while in our country there are only very few or shallow reports on the research of Rubus L. using molecular biological methods. By referring to relative materials, this paper summarized the application of Molecular markers of Rubus L., its main research field and the newest research progress in construction of genetic maps as well as gene identification of Rubus L. Besides, the applying future of its research was also discussed. It pointed out that the molecular methodologies were applied widely in many fields of biology of Rubus L. and good result has been reached, but most of the methodologies were still used with limitations. Therefore, for the success of the research it was the key to choose different and suitable methodologies according to research aims; and selecting appropriate means of molecular biology in its extensive and deep research is going to play an inestimable role in the production application and the protection of biodiversity of Rubus L.