To explore the effects of side-deep nitrogen reduction of slow mixed fertilizer on nitrogen use efficiency, yield and quality of rice, two different maturity cultivars were treated with blank (N0) treatment and conventional fertilizer treatment (240 kg/hm2, CFM) as control, four treatments of no reduction of nitrogen fertilizer (FM1), 7% reduction of nitrogen fertilizer (FM2), 14% reduction of nitrogen fertilizer (FM3) and 21% reduction of nitrogen fertilizer (FM4) were set for side-deep fertilization. The study aims to provide theoretical and technical guidance for the application of large-area mechanical inserted slow mixing fertilizer. The results showed that different fertilization treatments affected dry matter accumulation at different growth stages of rice, the dry matter accumulation of CFM treatment was significantly higher than that of other treatments in the middle and early growth stages (tillering stage to jointing stage), and FM1 treatment was significantly higher than that of other treatments from booting stage to maturity stage. The nitrogen content of rice plants also varied according to different growth stages. The nitrogen content of rice plants at tillering to jointing stage was in the order of CFM > FM1 > FM2 > FM3 > FM4 > N0, and that at booting to maturity stage was in the order of FM1, FM2 > CFM > FM3 > FM4 > N0. Under deep-side fertilization, moderate-reduced nitrogen had little effect on panicle number, and the grain number per panicle was the most in FM1 or FM2. The seed setting rate of deep-side fertilization was higher than that of the control, the 1000 grain weight and grain yield was higher in FM1 or FM2, and there were differences among different varieties; compared with the control, the deep-side fertilization treatment increased the nitrogen use efficiency. The nitrogen agronomic utilization rate was FM2>FM1 or FM3>CFM>FM4, and the nitrogen physiological utilization rate of CFM was the lowest, FM2 or FM3 was the highest. The nitrogen absorption utilization rate was FM2>FM1>FM3>CFM>FM4, and the nitrogen partial productivity was FM4>FM3>FM2>FM1>CFM. The treatments of side-deep fertilization is beneficial to reduce chalkiness grain rate, chalkiness and alkali elimination value, increase head rice rate, amylose content, protein content, gel consistency and disintegration value, and moderate-reduced nitrogen can improve rice quality. In production, it is suggested to adopt the side-deep fertilization technology of slow mixed fertilizer with nitrogen reduction of 7%-14%, which is beneficial to achieve nitrogen reduction, cost saving and high quality and yield of rice.