Abstract:

The aim of this study is to use plant tissue culture technology to screen out the most suitable medium for the introduced resource varieties (lines) SN6 and SN7 and self-breeding line YC24 of Cerasus humilis, and to determine the regeneration system of the three varieties, so as to provide technical support for the efficient and rapid large-scale propagation. Three C. humilis varieties were used as materials to study the disinfection methods of explants, the optimal medium for differentiation, proliferation and rooting and the ratio of hormones. The results showed that when the cephalosporin concentration was 100 mg/L, the explants had the highest survival rate and the lowest contamination rate. The optimal differentiation medium for SN6 and SN7 was MS+ 0.5 mg/L 6-BA+ 1.0 mg/L ZT+ 0.05 mg/L IBA, and the optimal differentiation medium for YC24 was MS+ 0.5 mg/L 6-BA+ 0.5 mg/L ZT+ 0.05 mg/L IBA. The optimal rooting medium of SN6 was 1/2MS+ 0.2 mg/L NAA+ 0.1 mg/L IBA+1.0 g/L activated carbon, and the optimal one-step rooting medium for SN7 and YC24 was 1/2MS+ 0.05 mg/L NAA+ 0.2 mg/L IBA. This study established a rapid propagation system for the shoots of C. humilis, and could lay a foundation for the subsequent transplanting, culture expanding and popularizing of the fine germplasms.

Key words: Cerasus humilis, endophytes, differentiation, rooting, cycocel, efficient regeneration